An ethanol-tolerant recombinant Escherichia coli expressing Zymomonas mobilis pdc and adhB genes for enhanced ethanol production from xylose

被引：0
作者：
Zhanchao Wang
Ming Chen
Yuquan Xu
Shuying Li
Wei Lu
Shuzhen Ping
Wei Zhang
Min Lin
机构：
[1] China Agricultural University,College of Biological Sciences
[2] Chinese Academy of Agricultural Science,Biotechnology Research Institute
来源：
Biotechnology Letters | 2008年 / 30卷
关键词：
Alcohol dehydrogenase; Ethanol; Pyruvate decarboxylase; Xylose;
D O I：
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中图分类号：
学科分类号：
摘要：
An ethanol-tolerant mutant, ET1, was isolated by an enrichment method from Escherichia coli JM109. Strains JM109 and ET1 were transformed with expression vector pZY507bc containing Zymomonas mobilis alcohol dehydrogenase II (adhB) and pyruvate decarboxylase (pdc) genes, resulting in an ethanol-sensitive recombinant strain JMbc and an ethanol-tolerant recombinant strain, ET1bc. Alcohol dehydrogenase and pyruvate decarboxylase activities were 24 and 32% lower, respectively, in JMbc than in ET1bc. ET1bc fermented 10% (w/v) xylose to give 39.4 g ethanol/l (77%, theoretical yield), a 1.3-fold increase compared with the ethanol-sensitive strain JMbc.
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页码：657 / 663
页数：6
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