Characterization of Genes Encoding Granule-Bound Starch Synthase in Sacred Lotus Reveals Phylogenetic Affinity of Nelumbo to Proteales

被引：0
作者：
Chao Gu
Lu Wang
Liyao Zhang
Yanling Liu
Mei Yang
Zhaonian Yuan
Shaohua Li
Yuepeng Han
机构：
[1] Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture,College of Life Science
[2] Wuhan Botanical Garden of the Chinese Academy of Sciences,College of Crop Science
[3] Wuhan University,undefined
[4] Key Laboratory of Aquatic Plant and Watershed Ecology,undefined
[5] Wuhan Botanical Garden,undefined
[6] Chinese Academy of Sciences,undefined
[7] Fujian Agricultural University,undefined
来源：
Plant Molecular Biology Reporter | 2013年 / 31卷
关键词：
Amylose; GBSS; Sacred lotus; Nelumbonaceae;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Starch is one of the main components of lotus rhizome and seed, and has a high potential influence on their eating and processing quality. However, little is known about the molecular mechanism underlying starch synthesis in lotus plants. Here, we report a genomewide identification and characterization of genes encoding granule-bound starch synthase in sacred lotus. Two GBSS genes, designated NnGBSSII-1 and NnGBSSII-2, have been identified in the genome of lotus variety “China Antique.” NnGBSSII-1 is expressed in all tested tissues, including leaf, flower, leafstalk, rhizome, and seed, whereas NnGBSSII-2 is a pseudogene and its transcripts have not been detected in any tissues. Overall, the expression level of NnGBSSII-1 gene is positively correlated with amylose accumulation in lotus rhizome and seed. Moreover, a phylogenetic analysis shows that GBSS genes from N. nucifera and N. lutea are clustered together, and they have a closer relationship with GBSS genes from Proteaceae. This result supports the hypothesis that Nelumbo is taxonomically placed in the lotus family, Nelumbonaceae, under the order Proteales. Our results will be helpful for future genetic improvement of starch quality in rhizome and lotus seed.
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页码：1157 / 1165
页数：8
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