Investigation of Chlamydiaceae in semen and cauda epididymidis and seroprevalence of Chlamydophila abortus in breeding bulls

被引：8
作者：
Karlsson A. [1 ,2 ]
Alenius S. [1 ]
Björkman C. [1 ]
Persson Y. [3 ]
Englund S. [4 ]
机构：
[1] Division of Ruminant Medicine and Veterinary Epidemiology, Department of Clinical Sciences, Swedish University of Agricultural Sciences (SLU), Faculty of Veterinary Medicine and Animal Science, SE-750 07, Uppsala
[2] Department of Animal Health, Section for Farm Animal Health and Welfare, National Veterinary Institute, N-0106 Oslo
[3] Department of Animal Health and Antimicrobial Strategies Section of Farm animals, National Veterinary Institute (SVA), SE-751 89, Uppsala
[4] Department of Animal Health and Antimicrobial Strategies Section of Antibiotics, National Veterinary Institute (SVA), SE-751 89, Uppsala
来源：
Acta Veterinaria Scandinavica | / 52卷 / 1期
关键词：
Semen Sample; Chlamydial Infection; Internal Amplification Control; Scrotal Circumference; Bull Semen;
D O I：
10.1186/1751-0147-52-2
中图分类号：
学科分类号：
摘要：
Background: Reproductive disorders associated with chlamydial infection have been reported worldwide in cattle and there are indications of potential venereal transmission.Methods: Semen samples from 21 dairy bulls and cauda epididymidis tissue samples from 43 beef bulls were analysed for chlamydial agent by real-time polymerase chain reaction (PCR) including an internal amplification control (mimic). Additionally, presence of antibodies against Chlamydophila (Cp.) abortus among the bulls was investigated with the commercial Pourquier® ELISA Cp. abortus serum verification kit.Results: No chlamydial agent was detected by PCR in either the semen samples or in the tissue samples. Additionally, no antibodies against Cp. abortus were detected.Conclusions: The results suggest that Cp. abortus is very rare, or absent in Swedish bulls and thus the risk for venereal transmission of chlamydial infection through their semen is low. However, because Chlamydophila spp. infection rates seem to differ throughout the world, it is essential to clarify the relative importance of transmission of the infection through semen on cattle fertility. © 2010 Karlsson et al; licensee BioMed Central Ltd.
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