Cloning, expression and characterization of a β-agarase gene from a marine bacterium, Pseudomonas sp. SK38

被引：0
|
作者：
Nam-Young Kang
Yong-Lark Choi
Young-Su Cho
Beom-Kyu Kim
Beong-Sam Jeon
Jae-Young Cha
Cherol-Ho Kim
Young-Choon Lee
机构：
[1] Dong-A University,Faculty of Biotechnology, College of Natural Resources and Life Science
[2] Dongguk University,Department of Biochemistry and Molecular Biology, College of Oriental Medicine
[3] Biotechnology Research Laboratory,undefined
[4] Biohub Co. Ltd.,undefined
来源：
Biotechnology Letters | 2003年 / 25卷
关键词：
agarase; cloning; expression;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A gene (pagA) encoding β-agarase from Pseudomonas sp. SK38 was cloned and expressed in Escherichia coli. The structural gene consists of 1011 bp encoding 337 amino acids with a predicted molecular weight of 37 326 and has a signal peptide of 18 amino acids. The deduced amino acid sequence showed 57% and 58% homology to β-agarase from Pseudoalteromonas atalntica and Aeromonas sp., respectively. The recombinant enzyme was purified and biochemically characterized. The enzyme had maximum activity at pH 9 and 30 °C. It was stable at pHs from 8 to 9 and below 37 °C.
引用
收藏
页码：1165 / 1170
页数：5
相关论文
共 50 条
  • [1] Cloning, expression and characterization of a β-agarase gene from a marine bacterium, Pseudomonas sp SK38
    Kang, NY
    Choi, YL
    Cho, YS
    Kim, BK
    Jeon, BS
    Cha, JY
    Kim, CH
    Lee, YC
    BIOTECHNOLOGY LETTERS, 2003, 25 (14) : 1165 - 1170
  • [2] Cloning of the cel9A gene and characterization of its gene product from marine bacterium Pseudomonas sp. SK38
    S. Ryu
    S. Cho
    S. Park
    W. Lim
    M. Kim
    S. Hong
    D. Bae
    Y. Park
    B. Kim
    H. Kim
    H. Yun
    Applied Microbiology and Biotechnology, 2001, 57 : 138 - 145
  • [3] Cloning of the ce/9A gene and characterization of its gene product from marine bacterium Pseudomonas sp SK38
    Ryu, SK
    Cho, SJ
    Park, SR
    Lim, WJ
    Kim, MK
    Hong, SY
    Bae, DW
    Park, YW
    Kim, BK
    Kim, H
    Yun, HD
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2001, 57 (1-2) : 138 - 145
  • [4] Cloning and expression of an agarase gene from a marine bacterium Pseudomonas sp. w7
    Jai-Yul Kong
    Sun-Hee Hwang
    Bong-Jo Kim
    Seoung-Kwon Bae
    Jong-Deog Kim
    Biotechnology Letters, 1997, 19 : 23 - 26
  • [5] Cloning and expression of an agarase gene from a marine bacterium Pseudomonas sp w7
    Kong, JY
    Hwang, SH
    Kim, BJ
    Bae, SK
    Kim, JD
    BIOTECHNOLOGY LETTERS, 1997, 19 (01) : 23 - 26
  • [6] Cloning of Agarase Gene from Non-Marine Agarolytic Bacterium Cellvibrio sp.
    Ariga, Osamu
    Inoue, Takayoshi
    Kubo, Hajime
    Minami, Kimi
    Nakamura, Mitsuteru
    Iwai, Michi
    Moriyama, Hironori
    Yanagisawa, Mitsunori
    Nakasaki, Kiyohiko
    JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 2012, 22 (09) : 1237 - 1244
  • [7] Cloning, expression and characterization of a new agarase-encoding gene from marine Pseudoalteromonas sp.
    Xinzhi Lu
    Yan Chu
    Qianqian Wu
    Yuchao Gu
    Feng Han
    Wengong Yu
    Biotechnology Letters, 2009, 31 : 1565 - 1570
  • [8] Cloning, expression and characterization of a new agarase-encoding gene from marine Pseudoalteromonas sp.
    Lu, Xinzhi
    Chu, Yan
    Wu, Qianqian
    Gu, Yuchao
    Han, Feng
    Yu, Wengong
    BIOTECHNOLOGY LETTERS, 2009, 31 (10) : 1565 - 1570
  • [9] Gene cloning, expression and characterization of a neoagarotetraose-producing β-agarase from the marine bacterium Agarivorans sp. HZ105
    Bokun Lin
    Guoyong Lu
    Yandan Zheng
    Wei Xie
    Shengkang Li
    Zhong Hu
    World Journal of Microbiology and Biotechnology, 2012, 28 : 1691 - 1697
  • [10] Gene cloning, expression and characterization of a neoagarotetraose-producing β-agarase from the marine bacterium Agarivorans sp HZ105
    Lin, Bokun
    Lu, Guoyong
    Zheng, Yandan
    Xie, Wei
    Li, Shengkang
    Hu, Zhong
    WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2012, 28 (04): : 1691 - 1697
12345