Use of the 2A sequence from foot-and-mouth disease virus in the generation of retroviral vectors for gene therapy

被引：0
作者：
P de Felipe
V Martín
M L Cortés
M Ryan
M Izquierdo
机构：
[1] Centro de Biología Molecular Severo Ochoa,Departamento de Bioquímica y Biología Molecular
[2] Universidad Autónoma de Madrid,Department of Biochemistry
[3] Facultad de Ciencias,undefined
[4] University of St Andrews,undefined
[5] St Andrews,undefined
来源：
Gene Therapy | 1999年 / 6卷
关键词：
bicistronic retroviral vectors; FMDV 2A; pac; HSV1tk; gene therapy;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
We describe the construction of retroviral plasmid vectors in which two genes are linked by a minimum of 96 nucleotides encoding the 2A sequence from the picornavirus foot-and-mouth disease virus (FMDV). Transcription and trans- lation gives rise to a bicistronic mRNA and two independent protein products. The system offers advantages to other alternative ways to create polycistronic mRNAs and can be used in gene therapy delivery vectors.
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页码：198 / 208
页数：10
相关论文
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