Efficiency of naphthalene and salicylate degradation by a recombinant Pseudomonas putida mutant strain defective in glucose metabolism

被引：0

作者：

S. Samanta

B. Bhushan

R. Jain

机构：

[1] Institute of Microbial Technology,

[2] Sector-39A,undefined

[3] Chandigarh-160 036,undefined

[4] India,undefined

[5] Present address: Department of Microbiology,undefined

[6] The University of Iowa,undefined

[7] 3–401 Bowen Science Building,undefined

[8] Iowa City,undefined

[9] IA 52242,undefined

[10] USA,undefined

[11] Present address: Department of Biochemistry and Molecular Biology,undefined

[12] Wayne State University,undefined

[13] School of Medicine,undefined

[14] 540 East Canfield,undefined

[15] Detroit,undefined

[16] MI 48201,undefined

[17] USA,undefined

来源：

Applied Microbiology and Biotechnology | 2001年 / 55卷

关键词：

Glucose; Carbon Source; Pseudomonas; Naphthalene; Glucose Metabolism;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Metabolically engineered microorganisms may have tremendous potential in removing toxic compounds from nature. In general, microorganisms prefer to utilize simpler carbon sources over toxic compounds when both are present in an environment and, therefore, the presence of simpler carbon sources may greatly reduce the efficiency of a microorganism towards toxic compounds. If a microorganism is prevented from utilizing simpler carbon sources, thereby making it totally dependent upon the toxic compounds, it should increase the specificity for and efficiency of degradation of the toxic compounds in the presence of other, simpler carbon sources. To test this hypothesis, the efficiency of naphthalene and salicylate degradation in the presence of glucose by a recombinant Pseudomonas putida strain mutated in glucose metabolism was determined and compared to the non-mutated strain. Results obtained indicate that the impairment of glucose metabolism leads to better degradation of naphthalene and salicylate in the presence of glucose.

引用

页码：627 / 631

页数：4