Extraction, characterization and antioxidant activities of an acidic polysaccharide from Dendrobium devonianum

被引：0
作者：
Yijun Fan
Mengchuan Lin
Aoxue Luo
机构：
[1] Chengdu Campus of Sichuan Agricultural University,
来源：
Journal of Food Measurement and Characterization | 2022年 / 16卷
关键词：
Acidic polysaccharide; Antioxidant ability; Enzyme-extraction; Structural characteristics;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Dendrobium devonianum Paxt (D. devonianum) is widely used as a healthy food and traditional Chinese medicine. It is rich in bioactive polysaccharides, but the acidic polysaccharide have not been reported. In this research, the enzyme-assisted extraction and structure characterizations of an acid polysaccharide (DDPp2) and its antioxidant activities were studied. The results showed the optimum extraction conditions were at enzyme concentration of 0.94% at 51.06 °C and pH 4.94. The molecular weight of DDPp2 was 306 kDa and composed of four monosaccharides in the molar ratio of mannose:glucose:galactose:glucuronic acid = 6.37:4.99:1.71:2.39 with α-configuration sugar. SEM and TEM analysis showed that the surface of the polysaccharide was smooth and holes in the sheet and multiple molecular chains were intertwined with each other. Free radical scavenging tests in vitro showed DDPp2 had very strong scavenging abilities for hydroxyl radical, ABTS radical and superoxide anion free radicals. Cell experiment showed DDPp2 had no cytotoxicity and could significantly increase the activities of CAT, SOD and GPX, reduce the content of MDA in RAW264.7 cells. The investigation showed that DDPp2 is a potential antioxidant.
引用
收藏
页码：867 / 879
页数：12
相关论文
共 244 条
  • [31] Zhang H(2020) Lindl Int. J. Biol. Macromol. 153 942-549
  • [32] Zhu J(2018)Composition analysis and antioxidant activity of polysaccharide from Int. J. Biol. Macromol. 118 816-304
  • [33] Ren FZ(2013)A novel polysaccharide from Food Sci. 34 6-38
  • [34] Liu YG(2017) serves as a TLR4 agonist for activating macrophages Mod Food Technol. 033 72-350
  • [35] Yang LL(2008)Enzyme-assisted extraction of polysaccharides from Carbohydr. Polym. 71 544-1445
  • [36] Zhang Y(1978) and its functional properties and immunomodulatory activity Carbohydr. Res. 67 295-57
  • [37] Liu XC(1979)Polysaccharides from hot water extracts of roasted Coffea arabica beans: Isolation and characterization J. Raman Spectrosc. 8 35-78
  • [38] Wu ZJ(2008)Structure of an exocellular polysaccharide of Carbohydr. Polym. 73 344-undefined
  • [39] Gilbert RG(2005) TN-4, a spontaneous mutant strain of Int. Immunopharmacol. 5 1436-undefined
  • [40] Deng B(2020) TY1-2 Carbohydr. Polym. 246 116551-undefined
12345678910