Deposition, turnover, and release of CENH3 at Arabidopsis centromeres

被引：0
作者：
Inna Lermontova
Twan Rutten
Ingo Schubert
机构：
[1] Leibniz Institute of Plant Genetics and Crop Plant Research (IPK),
来源：
Chromosoma | 2011年 / 120卷
关键词：
Mature Leaf; Fluorescence Recovery After Photobleaching; Cell Cycle Stage; Enhanced Yellow Fluorescent Protein; Fluorescence Recovery After Photobleaching Experiment;
D O I：
暂无
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学科分类号：
摘要：
The kinetochore is a complex multiprotein structure located at centromeres and required for the proper segregation of chromosomes during mitosis and meiosis. An important role in kinetochore assembly and function plays the centromeric histone H3 variant (CENH3). Cell cycle stage of CENH3 deposition to centromeres varies between different organisms. We confirmed by in vivo studies that deposition of Arabidopsis CENH3 takes place at centromeres during G2 and demonstrated that additionally a low turnover of CENH3 occurs along the cell cycle, apparently for replacement of damaged protein. Furthermore, enhanced yellow fluorescent protein (EYFP)-CENH3 of photobleached chromocenters is not replaced by EYFP-CENH3 molecules from unbleached centromeres of the same nucleus, indicating a stable incorporation of CENH3 into centromeric nucleosomes. In differentiated endopolyploid nuclei however, the amount of CENH3 at centromeres declines with age.
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页码：633 / 640
页数：7
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