DNA-binding and photocleavage, cytotoxicity, apoptosis and antioxidant activity studies of ruthenium(II) complexes

被引:0
作者
Li Xu
Yang-Yin Xie
Nan-Jing Zhong
Zhen-Hua Liang
Juan He
Hong-Liang Huang
Yun-Jun Liu
机构
[1] Guangdong Pharmaceutical University,School of Chemistry and Chemical Engineering
[2] Guangdong Pharmaceutical University,School of Pharmacy
[3] Guangdong Pharmaceutical University,School of Food Science
[4] Guangdong Pharmaceutical University,School of Life Science and Biopharmacological
来源
Transition Metal Chemistry | 2012年 / 37卷
关键词
Ruthenium; Phen; Acridine Orange; Ruthenium Complex; Acridine Orange;
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摘要
Two ruthenium(II) polypyridyl complexes, namely [Ru(phen)2(DMDPPZ)](ClO4)21 (phen = 1,10-phenanthroline, DMDPPZ = 3,6-dimethyldipyrido[3,2-a:2′,3′-c]phenazine) and [Ru(dmp)2(DMDPPZ)](ClO4)22 (dmp = 2,9-dimethyl-1,10-phenanthroline), have been synthesized and characterized. The DNA-binding properties of the complexes were investigated by spectrophotometric methods, viscosity measurements, and photoactivated cleavage studies. The DNA-binding constants for complexes 1 and 2 have been determined as 8.78 (±0.94) × 105 M−1 (s = 3.02) and 1.26 (±0.35) × 105 M−1 (s = 1.69), respectively. The results suggest that these complexes bind to calf thymus DNA through intercalation. When irradiated at 365 nm, the complexes promote the photocleavage of pBR322 DNA, and complex 1 cleaves DNA more effectively than complex 2 under comparable experimental conditions. The cytotoxicities of complexes 1 and 2 have been evaluated by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide) method. Complex 2 shows higher anticancer potency than complex 1 against four tumor cell lines. The apoptosis-inducing activity was assessed by acridine orange/ethidium bromide staining assay, and the antioxidant activities of these complexes against hydroxyl radical were also explored.
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页码:197 / 205
页数:8
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