Luminescent conjugated oligothiophenes distinguish between α-synuclein assemblies of Parkinson’s disease and multiple system atrophy

被引：0
作者：
Therése Klingstedt
Bernardino Ghetti
Janice L. Holton
Helen Ling
K. Peter R. Nilsson
Michel Goedert
机构：
[1] MRC Laboratory of Molecular Biology,Department of Pathology and Laboratory Medicine
[2] Indiana University School of Medicine,Department of Physics, Chemistry and Biology
[3] Queen Square Brain Bank,undefined
[4] UCL Queen Square Institute of Neurology,undefined
[5] Linköping University,undefined
来源：
Acta Neuropathologica Communications | / 7卷
关键词：
α-Synuclein; Luminescent conjugated oligothiophene; Multiple system atrophy; Neurodegeneration; Parkinson’s disease; Spectral analysis;
D O I：
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摘要：
Synucleinopathies [Parkinson’s disease with or without dementia, dementia with Lewy bodies and multiple system atrophy] are neurodegenerative diseases that are defined by the presence of filamentous α-synuclein inclusions. We investigated the ability of luminescent conjugated oligothiophenes to stain the inclusions of Parkinson’s disease and multiple system atrophy. They stained the Lewy pathology of Parkinson’s disease and the glial cytoplasmic inclusions of multiple system atrophy. Spectral analysis of HS-68-stained inclusions showed a red shift in multiple system atrophy, but the difference with Parkinson’s disease was not significant. However, when inclusions were double-labelled for HS-68 and an antibody specific for α-synuclein phosphorylated at S129, they could be distinguished based on colour shifts with blue designated for Parkinson’s disease and red for multiple system atrophy. The inclusions of Parkinson’s disease and multiple system atrophy could also be distinguished using fluorescence lifetime imaging. These findings are consistent with the presence of distinct conformers of assembled α-synuclein in Parkinson’s disease and multiple system atrophy.
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