Photoactivatable retroviral vectors: a strategy for targeted gene delivery

被引：0

作者：

MW Pandori

T Sano

机构：

[1] The Center for Molecular Imaging Diagnosis and Therapy and Basic Science Laboratory,Department of Radiology

[2] Beth Israel Deaconess Medical Center,undefined

[3] Harvard Medical School,undefined

来源：

Gene Therapy | 2000年 / 7卷

关键词：

retrovirus; biotin; photocleavable; gene therapy;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

We have explored a novel strategy for the targeting of retroviral vectors to particular sites or cell types. This strategy involves a method whereby the infectivity of a retroviral vector is neutralized by treatment of viral particles with a photocleavable, biotinylation reagent. These modified viral vectors possess little to no infectivity for target cells. Exposure of these modified viral vectors to long-wavelength UV light induces a reversal of the neutralizing, chemical modification resulting in restoration of infectivity to the viral vector. This infectivity ‘trigger’ possesses great potential, both as a research tool and as a novel tactic for the targeting of gene-transfer agents, since it would become possible to direct both the time and location of a viral infection in a versatile manner.

引用

页码：1999 / 2006

页数：7