Effects of elicitation on the production of saponin in cell culture of Panax ginseng

被引：9

作者：

Denbow M.L. ^{[1
]}

Eckersley R. ^{[2
]}

Welsh A.W. ^{[1
]}

Taylor M.J. ^{[1
]}

Carter R.C. ^{[3
]}

Cosgrove D.O. ^{[2
]}

Fisk N.M. ^{[1
]}

机构：

[1] Centre for Fetal Care, Institute of Obstetrics and Gynaecology, Imperial College School of Medicine, Queen Charlotte’s and Chelsea Hospital, London

[2] Department of Imaging, Imperial College School of Medicine, Hammersmith Hospital, Burgess Hill

[3] Schering Health Care Limited, West Sussex

来源：

Plant Cell Reports | 2001年 / 20卷 / 07期

关键词：

Elicitor; Panax ginseng; Saponin;

D O I：

10.1007/s002990100378

中图分类号：

学科分类号：

摘要：

This study was initiated to investigate the impacts of elicitor concentration and elicitor-adding time on the saponin synthesis and the cell growth of Panax ginseng cell suspensions. Both of the elicitors tested, yeast extract and methyl jasmonate, significantly improved saponin production. The highest additive level of the seven ginsenosides tested was 2.07% (dry weight basis), which was 28-fold higher than that in the control. The optimum time to add either elicitor was found to be on the day of inoculation. The addition of either elicitor did not show as significant an influence on cell growth as on saponin production. It was advisable to remove 2,4-dichlorophenoxyacetic acid (2,4-D) from the medium when methyl jasmonate was used as the elicitor as methyl jasmonate interacts antagonistically with 2,4-D. These results suggest that the addition of an elicitor to ginseng cell suspension cultures could stimulate saponin production.

引用

页码：674 / 677

页数：3

共 17 条

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