Mitotic recombination and localized DNA double-strand breaks are induced after 8-methoxypsoralen and UVA irradiation in Saccharomyces cerevisiae

被引：0

作者：

Dardalhon M. ^{[1
]}

De Massy B. ^{[2
]}

Nicolas A. ^{[2
]}

Averbeck D. ^{[1
]}

机构：

[1] Institut Curie Section de Recherche, UMR 218 CNRS, LRC no. I du CEA, F-75248 Paris Cedex 05, 26, rue d'Ulm

[2] Institut Curie Section de Recherche, UMR 144 CNRS, F-75248-Paris Cedex 05

来源：

Current Genetics | 1998年 / 34卷 / 1期

关键词：

8-Methoxypsoralen plus UVA; DNA double-strand breaks; Mitotic recombination; Saccharomyces cerevisiae;

D O I：

10.1007/s002940050363

中图分类号：

学科分类号：

摘要：

Mitotic recombination within the ARG4 gene of Saccharomyces cerevisiae was analysed after treatment of cells with the recombinogenic agent 8-methoxypsoralen (8-MOP) plus UVA. The appearance of DNA double-strand breaks (DSBs) in the ARG4 region during post-treatment incubation was also tested. The results obtained after 8-MOP plus UVA treatment indicate that in mitotic cells: (1) recombination at the ARG4 locus is increased 30-500 fold per survivor depending on the strains and the doses employed, (2) the increase of recombination results essentially from gene conversion events which involve the RV site located in the 5' region of the ARG4 gene twice as often as the Bgl site at the 3' end, (3) depending on 8-MOP/UVA dose, ectopic gene conversion is associated with reciprocal translocation, (4) DSBs occur preferentially in the ARG 5' region during post-treatment incubation, as well as in other intergenic regions containing both promoters or/and terminators of transcription, and (5) changes in sequence content in the 5' region of ARG4, which influences positions and frequencies of DSBs formed during repair, are correlated with a modification of the local chromatin structure.

引用

页码：30 / 42

页数：12

共 47 条

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