Segregation of molecules at cell division reveals native protein localization

被引：0

作者：

Landgraf D. ^{[1
]}

Okumus B. ^{[1
]}

Chien P. ^{[2
,3
]}

Baker T.A. ^{[2
]}

Paulsson J. ^{[1
]}

机构：

[1] Department of Systems Biology, Harvard Medical School, Boston, MA

[2] Department of Biology, Massachusetts Institute of Technology, Cambridge, MA

[3] Department of Biochemistry and Molecular Biology, University of Massachusetts Amherst, Amherst, MA

来源：

Nature Methods | 2012年 / 9卷 / 5期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1038/nmeth.1955

中图分类号：

学科分类号：

摘要：

We introduce a nonintrusive method exploiting single-cell variability after cell division to validate protein localization. We found that Clp proteases, widely reported to form biologically relevant foci, were uniformly distributed in Escherichia coli cells, and that many commonly used fluorescent proteins caused severe mislocalization when fused to homo-oligomers. Retagging five other reportedly foci-forming proteins with the most monomeric fluorescent protein tested suggests that the foci were caused by the fluorescent tags. © 2012 Nature America, Inc. All rights reserved.

引用

页码：480 / 482

页数：2

共 19 条

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