Interaction between the keratin cytoskeleton and eEF1Bγ affects protein synthesis in epithelial cells

被引：0
|
作者：
Seyun Kim
Juliane Kellner
Chang-Hun Lee
Pierre A Coulombe
机构：
[1] The Johns Hopkins University School of Medicine,Department of Biological Chemistry
[2] The Johns Hopkins University School of Medicine,Department of Dermatology
来源：
Nature Structural & Molecular Biology | 2007年 / 14卷
关键词：
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Eukaryotic elongation factor-1 (eEF1) is essential for peptide-chain elongation during translation. We report that its γ subunit (eEF1Bγ) specifically binds, and bundles, keratin intermediate filaments. Disrupting this interaction depresses translation by ∼20% and selectively increases 80S ribosomes in epithelial cells, an outcome recapitulated by RNA interference–mediated silencing of eEF1Bγ. These findings extend the emerging relationship between keratin proteins and the translational machinery.
引用
收藏
页码：982 / 983
页数：1
相关论文
共 50 条
  • [31] Characterization of the interaction between Yersinia enterocolitica biotype 1A and phagocytes and epithelial cells in vitro
    Grant, T
    Bennett-Wood, V
    Robins-Browne, RM
    INFECTION AND IMMUNITY, 1999, 67 (09) : 4367 - 4375
  • [32] Regulated protein-protein interaction between aPKC and PAR-3 plays an essential role in the polarization of epithelial cells
    Nagai-Tamai, Y
    Mizuno, K
    Hirose, T
    Suzuki, A
    Ohno, S
    GENES TO CELLS, 2002, 7 (11) : 1161 - 1171
  • [33] Protein-protein interaction of retinoic acid receptor α and thyroid transcription factor-1 in respiratory epithelial cells
    Yan, C
    Naltner, A
    Conkright, J
    Ghaffari, M
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (24) : 21686 - 21691
  • [34] EFFECT OF ULTRAVIOLET-B RADIATION ON PROTEIN-SYNTHESIS IN CULTURED LENS EPITHELIAL-CELLS
    ANDLEY, UP
    WALSH, A
    KOCHEVAR, IE
    REDDAN, JR
    CURRENT EYE RESEARCH, 1990, 9 (11) : 1099 - 1106
  • [35] MODULATION OF MILK PROTEIN-SYNTHESIS THROUGH ALTERATION OF THE CYTOSKELETON IN MOUSE MAMMARY EPITHELIAL-CELLS CULTURED ON A RECONSTITUTED BASEMENT-MEMBRANE
    SEELY, KA
    AGGELER, J
    JOURNAL OF CELLULAR PHYSIOLOGY, 1991, 146 (01) : 117 - 130
  • [36] INTERACTION BETWEEN AFLATOXIN-B1 (AFB1) AND OXYTETRACYCLINE (OTC) ON PROTEIN AND RNA-SYNTHESIS IN ISOLATED RAT HEPATOCYTES
    BLAUDE, MN
    DEPELCHIN, B
    ANSAY, M
    KRACK, G
    ROBERFROID, M
    VETERINARY AND HUMAN TOXICOLOGY, 1987, 29 : 103 - 104
  • [37] Protein tyrosine phosphatase 1B regulates the activity of retinal pigment epithelial cells
    Du, Zhao-dong
    Hu, Li-ting
    Zhao, Gui-qiu
    Li, Ying
    Ma, Zhi-zhong
    MOLECULAR VISION, 2015, 21 : 523 - 531
  • [38] The PDZ protein-binding domain of GLUT1 affects dynamics of vesicular targeting in mammary epithelial cells
    Haney, PM
    PEDIATRIC RESEARCH, 2004, 55 (04) : 384A - 384A
  • [39] Bicuculline regulated protein synthesis is dependent on Homer1 and promotes its interaction with eEF2K through mTORC1-dependent phosphorylation
    Gladulich, Luis F. H.
    Xie, Jianling
    Jensen, Kirk B.
    Kamei, Makoto
    Paes-de-Carvalho, Roberto
    Cossenza, Marcelo
    Proud, Christopher G.
    JOURNAL OF NEUROCHEMISTRY, 2021, 157 (04) : 1086 - 1101
  • [40] Interaction of PDZRhoGEF with microtubule-associated protein 1 light chains - Link between microtubules, actin cytoskeleton, and neuronal polarity
    Longhurst, DM
    Watanabe, M
    Rothstein, JD
    Jackson, M
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (17) : 12030 - 12040
12345