Incubation of sperm heads impairs fertilization and early embryo development following intracytoplasmic sperm injection (ICSI) by decreasing oocyte activation in mice

被引:0
作者
Hui Long
Sheng-Sheng Lu
Yan-Ping Kuang
Zhi-Guang Yan
Hong-Xing Liang
Sha Yu
Wei-Ran Chai
Zheng Yan
Qi-Feng Lyu
机构
[1] Shanghai Ninth People’s Hospital Affiliated Shanghai Jiao Tong University School of Medicine,Department of Assisted Reproduction
[2] Guangxi University,State Key Laboratory for Conservation and Utilization of Subtropical Agro
[3] Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Bioresources/College of Animal Science and Technology
来源
Biotechnology Letters | 2013年 / 35卷
关键词
DNA integrity; Embryo development; Fertilization; Intracytoplasmic sperm injection; Sperm head;
D O I
暂无
中图分类号
学科分类号
摘要
When intracytoplasmic sperm injection (ICSI) is performed in mice, isolation of sperm heads is usually performed prior to injections in order to increase the efficiency of the procedure. Consequently, the isolated sperm heads undergo an inevitable incubation in vitro. However, little is known about the effects of this incubation step on fertilization and embryo development following ICSI. When we incubated sperm heads at 37 °C, there was a significant time-dependent decrease in fertilization and blastocyst formation. Moreover, the DNA integrity of the sperm heads was maintained over 12 h incubation. Using assisted oocyte activation, these defects in fertilization and embryo development were rescued. Taken together, incubation of sperm heads following isolation can affect the oocyte-activating capacity of sperm thereby compromising fertilization and embryo development associated with ICSI.
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页码:1823 / 1829
页数:6
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