Incubation of sperm heads impairs fertilization and early embryo development following intracytoplasmic sperm injection (ICSI) by decreasing oocyte activation in mice

被引：0
作者：
Hui Long
Sheng-Sheng Lu
Yan-Ping Kuang
Zhi-Guang Yan
Hong-Xing Liang
Sha Yu
Wei-Ran Chai
Zheng Yan
Qi-Feng Lyu
机构：
[1] Shanghai Ninth People’s Hospital Affiliated Shanghai Jiao Tong University School of Medicine,Department of Assisted Reproduction
[2] Guangxi University,State Key Laboratory for Conservation and Utilization of Subtropical Agro
[3] Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Bioresources/College of Animal Science and Technology
来源：
Biotechnology Letters | 2013年 / 35卷
关键词：
DNA integrity; Embryo development; Fertilization; Intracytoplasmic sperm injection; Sperm head;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
When intracytoplasmic sperm injection (ICSI) is performed in mice, isolation of sperm heads is usually performed prior to injections in order to increase the efficiency of the procedure. Consequently, the isolated sperm heads undergo an inevitable incubation in vitro. However, little is known about the effects of this incubation step on fertilization and embryo development following ICSI. When we incubated sperm heads at 37 °C, there was a significant time-dependent decrease in fertilization and blastocyst formation. Moreover, the DNA integrity of the sperm heads was maintained over 12 h incubation. Using assisted oocyte activation, these defects in fertilization and embryo development were rescued. Taken together, incubation of sperm heads following isolation can affect the oocyte-activating capacity of sperm thereby compromising fertilization and embryo development associated with ICSI.
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页码：1823 / 1829
页数：6
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共 110 条
  • [1] Bungum M(2004)The predictive value of sperm chromatin structure assay (SCSA) parameters for the outcome of intrauterine insemination, IVF and ICSI Hum Reprod 19 1401-1408
  • [2] Humaidan P(2008)The roles of Ca Dev Biol 315 257-279
  • [3] Spano M(2005), downstream protein kinases, and oscillatory signaling in regulating fertilization and the activation of development Fertil Steril 84 130-140
  • [4] Jepson K(2012)Sperm DNA fragmentation negatively correlates with velocity and fertilization rates but might not affect pregnancy rates Adv Exp Med Biol 740 1095-1121
  • [5] Bungum L(2004)Calcium oscillations, oocyte activation, and phospholipase C zeta J Biol Chem 279 10408-10412
  • [6] Giwercman A(1996)Recombinant phospholipase Czeta has high Ca Biol Reprod 55 789-795
  • [7] Ducibella T(2010) sensitivity and induces Ca Reprod Biomed Online 21 663-666
  • [8] Fissore R(2006) oscillations in mouse eggs Proc Natl Acad Sci USA 103 17661-17666
  • [9] Huang CC(2011)Fertilization and development of mouse oocytes injected with isolated sperm heads Reproduction 142 285-293
  • [10] Lin DP(2012)New technique for mouse oocyte injection via a modified holding pipette BioEssays 34 126-134
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