Use of optical-spectral methods for in vivo noninvasive assessment of nanoparticles accumulation in biological tissues

Methods for noninvasive estimation of the nanoparticles concentration in biological tissues in vivo were developed with a view to laying the scientific groundwork for diagnosis and treatment of malignant tumors. The methods were tested in experimental animals (mice inoculated with Ehrlich carcinoma). Luminescent spectroscopy and diffuse reflectance spectroscopy were applied for examination of biotissues. A special algorithm of spectral data analysis with the use of the model solution simulating the optical properties of biotissues was devised. It was shown that the optical-spectral methods developed are effective in high-accuracy noninvasive real-time quantitative analysis of the nanoparticles accumulation in biotissues.