Heterogeneity of p53 mutational status in the superficial spreading type of early gastric carcinoma

被引：21

作者：

Iwamatsu H. ^{[1
,2
]}

Nishikura K. ^{[2
]}

Watanabe H. ^{[2
]}

Ajioka Y. ^{[2
]}

Hashidate H. ^{[2
]}

Kashimura H. ^{[1
]}

Asakura H. ^{[1
]}

机构：

[1] Third Department of Internal Medicine, Niigata University, School of Medicine, Niigata

[2] First Department of Pathology, Niigata University, School of Medicine, Niigata 951-8510

来源：

Gastric Cancer | 2001年 / 4卷 / 1期

关键词：

Clonal selection; Clonality; Heterogeneity; p53; Gene; Superficial spreading type of gastric carcinoma;

D O I：

10.1007/s101200100012

中图分类号：

学科分类号：

摘要：

Background. The superficial spreading type of gastric carcinoma may originate from either a single cellular clone or from several different clones; this issue remains controversial. Indeed, the p53 gene has been shown to play an important role in gastric carcinogenesis, but there have been only a few reports on the heterogeneity of gastric carcinoma with respect to the p53 gene. Methods. We analyzed seven cases of the superfcial spreading type of gastric submucosal carcinomas (80 lesions; 10 to 17 per case) which showed different histological types and/or different p53 protein staining patterns. Direct sequences of polymerase chain reaction products were used for the analysis. Results. p53 Gene heterogeneity in mucosal carcinoma lesions was detected in three cases. However, in all of the cases, the p53 mutational pattern was identical to that found in the submucosal carcinoma lesions. In the heterogeneous cases, the mutation in the submucosal carcinoma was one of the mutation patterns found among the mucosal carcinoma lesions. More precisely, the mutational pattern of both submucosal carcinoma lesions and the mucosal lesions located just above them, was identical. Conclusion. These data suggest that, with regard to the p53 gene, in some superficial spreading types of gastric carcinomas, there are various subclones in the mucosal carcinoma; one of these subclones becomes predominant through clonal selection, and, thus, invades the submucosa.

引用

页码：20 / 26

页数：6

共 28 条

[1]

Stout A.P., Superficial spreading type of carcinoma of the stomach, Arch Surg, 44, pp. 651-657, (1942)

[2]

Kitamura K., Yamaguchi T., Okamoto K., Nishida T., Takahashi T., Superficial spreading type of early gastric cancer, Br J Cancer, 74, pp. 1834-1837, (1996)

[3]

Tamura G., Kihara T., Nomura K., Terada M., Sugimura T., Hirohashi S., Detection of frequent p53 gene mutations in primary gastric cancer by cell sorting and polymerase chain reaction single-strand conformation polymorphism analysis, Cancer Res, 51, pp. 3056-3058, (1991)

[4]

Yokozaki H., Kuniyas H., Kitadai Y., Nishimura K., Toda H., Ayhan A., Et al., p53 Point mutations in primary human gastric carcinomas, J Cancer Res Clin Oncol, 119, pp. 67-70, (1992)

[5]

Imazeki F., Omata M., Nose H., Ohta M., Isono K., p53 Gene mutations in gastric and esophageal cancers, Gastroenterology, 103, pp. 892-896, (1992)

[6]

Renault B., Broek M., Fodde R., Wijnen J., Pellegata N.S., Amadori D., Et al., Base transitions are the most frequent p53 genetic changes in gastric cancer, Cancer Res, 53, pp. 2614-2617, (1993)

[7]

Uchino S., Noguchi M., Ochiai A., Saito T., Kobayashi M., Hirohashi S., p53 Mutation in gastric cancer: A genetic model for carcinogenesis is common to gastric and colorectal cancer, Int J Cancer, 54, pp. 859-864, (1993)

[8]

Strickler J.G., Zheng J., Shu Q., Burgart L.J., Alberts S.R., Shibata D., p53 Mutations and microsatellite instability in sporadic gastric cancer: When guardians fail, Cancer Res, 54, pp. 4750-4755, (1994)

[9]

Ranzani G.N., Leinetti O., Padovan L.S., Calistri D., Renault B., Burrel M., Et al., p53 Gene mutations and protein nuclear accumulation are early events in intestinal type gastric cancer but late events in diffuse type, Cancer Epidemiol Biomarkers Prev, 4, pp. 223-231, (1995)

[10]

Bereanice H., Lim G., Soong R., Grieu F., Robbins P.D., House A.K., Iacopetta B.J., p53 Accumulation and mutation are prognostic indicators of poor survival in human gastric carcinoma, Int J Cancer, 69, pp. 200-204, (1996)

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