Heterologous expression in E. coli and functional characterization of the tomato CPR enzymes

被引：0
作者：
Won Choi
Seo Young Park
Hyun Min Kim
Thanh Dat Mai
Ju Hui Do
Hye Min Jang
Hyeon Bae Hwang
Eun Gyeong Song
Jae Sung Shim
Young Hee Joung
机构：
[1] Chonnam National University,School of Biological Sciences and Technology
来源：
Applied Biological Chemistry | / 66卷
关键词：
NADPH-cytochrome P450 reductase; Heterologous expression; Enzymatic characterization;
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摘要：
NADPH-cytochrome P450 reductase (CPR) is a key enzyme transferring electrons to cytochrome P450. In tomatoes (Solanum lycopersicum), two CPR genes, SlCPR1 and SlCPR2, were identified. In all the tested tomato tissues, SlCPR2 showed higher expression levels than SlCPR1. SlCPR2 expression increased significantly with jasmonic acid treatment. No significant changes were observed with salicylic acid or drought stress treatment. The cDNA of SlCPRs were expressed in Escherichia coli without any amino acid modification. And the heterologously expressed SlCPR enzymes were reacted with several protein and chemical substrates. SlCPR2 was more active than SlCPR1. Both SlCPR1 and SlCPR2 exhibited strong activity across a pH range of 6.0 to 9.0, with peak activity at pH 8.0. The study opens possibilities for CPR control, biocatalyst development, and exploring oxidase enzyme functions.
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