Trypsin-induced hemagglutination activity of porcine epidemic diarrhea virus

被引：0
作者：
Jung-Eun Park
Deu John M. Cruz
Hyun-Jin Shin
机构：
[1] Chungnam National University,Laboratory of Infectious Disease, College of Veterinary Medicine
[2] Research Institute of Veterinary Medicine,undefined
来源：
Archives of Virology | 2010年 / 155卷
关键词：
Porcine Epidemic Diarrhea Virus; Infectious Bronchitis Virus; Rabbit Erythrocyte; Neuraminidase Treatment; Porcine Epidemic Diarrhea Virus Strain;
D O I：
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中图分类号：
学科分类号：
摘要：
The hemagglutination (HA) activity of porcine epidemic diarrhea virus (PEDV) was investigated. Two cell-adapted strains of PEDV (KPEDV-9 and SM98LVec) were subjected to HA test against erythrocytes of various origin. Both strains showed HA activity with rabbit erythrocytes only after treatment with trypsin or neuraminidase. Optimal conditions for inducing HA activity of PEDV were 2 h incubation at 37°C using phosphate-buffered saline containing 0.1% BSA. These results suggest that the HA activity of PEDV is most likely caused by proteolytic action on it, which could be developed as a new diagnostic method to rapidly detect and differentiate PEDV infections from other enteric diseases.
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页码：595 / 599
页数：4
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