Simple bioseparations using self-cleaving elastin-like polypeptide tags

被引：0
|
作者：
Mahmoud Reza Banki
Liang Feng
David W Wood
机构：
[1] A213 E-QUAD,Department of Chemical Engineering
[2] Princeton University,Department of Molecular Biology
[3] Princeton University,undefined
来源：
Nature Methods | 2005年 / 2卷
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摘要：
We introduce a new method for the purification of recombinant proteins expressed in Escherichia coli using self-cleaving elastin-like polypeptide (ELP) fusion tags without the need for affinity chromatography or proteolytic tag removal. Using this method we obtained high purity, activity and reasonable yields for ten diverse target proteins.
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页码：659 / 662
页数：3
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