A novel compound-primed multiplex ARMS-PCR (CPMAP) for simultaneous detection of common PAH gene mutations

被引：0
作者：
Maryam Shaykholeslam Esfahani
Ehsan Shaykholeslam Esfahani
Sadeq Vallian
机构：
[1] University of Isfahan,Division of Genetics, Department of Biology, Faculty of Science
[2] Isfahan University of Technology,Division of Biotechnology, Department of Agricultural Biotechnology, Faculty of Agriculture
来源：
Metabolic Brain Disease | 2018年 / 33卷
关键词：
Multiplex ARMS-PCR; Compound primer; Phenylalanine hydroxylase mutations; Phenylketonuria;
D O I：
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中图分类号：
学科分类号：
摘要：
In this study, we introduce a novel compound-primed multiplex ARMS PCR (CPMAP) for simultaneous detection of common PAH gene mutations. This approach was used successfully for simultaneous identification of six most common PAH gene mutations in 137 phenylketonuria patients in the Iranian population. A total of six normal and six mutant allele-specific primers and 4 common primers containing a tag sequence of 12 base pair at the 5ˊ-end were designed and used in two separate optimized multiplex ARMS reactions followed by hot-start PCR. The products were separated and visualized on 3% agarose gel. The CPMAP genotyping data were completely in accordance with the direct sequencing results. The CPMAP suggests a reliable, economical and rapid method for simultaneous detection of PAH point mutations using conventional PCR, which could be applied for diagnosis of other gene mutations.
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页码：1165 / 1173
页数：8
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