Novel role of NADPH oxidase in ischemic myocardium: a study with Nox2 knockout mice

被引：0

作者：

Mahesh Thirunavukkarasu

Ram Sudheer Adluri

Bela Juhasz

Samson Mathews Samuel

Lijun Zhan

Anupinder Kaur

Gautam Maulik

Juan A Sanchez

Janet Hager

Nilanjana Maulik

机构：

[1] University of Connecticut School of Medicine,Molecular Cardiology and Angiogenesis Laboratory, Department of Surgery

[2] University of Debrecen,Department of Pharmacology

[3] University of Connecticut School of Medicine,Translational Genomics Core, Department of Genetics and Developmental Biology

[4] Harvard Medical School,Department of Radiology

[5] Saint Mary’s Hospital,Department of Surgery

来源：

Functional & Integrative Genomics | 2012年 / 12卷

关键词：

Ischemic preconditioning; Nox2; NADPH oxidase; Gene expression; Cardioprotection;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Several potential sources of reactive oxygen species (ROS) in cells exist. One source is NADPH oxidase, which is especially important for superoxide radical production. Nox2 is a primary regulatory subunit of NADPH oxidase. In the present study, we examined the role of ROS and NADPH oxidase in ischemic preconditioning (IP)-mediated cardioprotection by using Nox2−/− mice. Both wild-type (WT) and Nox2−/− mice were subjected to either 30 min of ischemia followed by 2 h of reperfusion (IR) or IP prior to 30 min ischemia and 2 h of reperfusion. Reduction in left ventricular developed pressure (60.1 versus 63 mmHg), dp/dtmax (893 versus 1,027 mmHg/s), and aortic flow (0.9 versus 1.8 ml/min) was observed in Nox2−/−IPIR compared to WTIPIR along with increased infarct size (33% versus 22%) and apoptosis after 120 min of reperfusion. Differentially regulated genes were demonstrated by comparing gene expression in WTIPIR versus Nox2−/− IPIR hearts. Selected differentially regulated genes such as β-catenin, SRPK3, ERDR1, ACIN1, Syntaxin-8, and STC1 were validated by real-time PCR. Taken together, this is the first report identifying important, differentially expressed genes during ischemic preconditioning in Nox2−/− mice by using microarray analysis.

引用

页码：501 / 514

页数：13

共 274 条

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