Novel role of NADPH oxidase in ischemic myocardium: a study with Nox2 knockout mice

被引：0

作者：

Mahesh Thirunavukkarasu

Ram Sudheer Adluri

Bela Juhasz

Samson Mathews Samuel

Lijun Zhan

Anupinder Kaur

Gautam Maulik

Juan A Sanchez

Janet Hager

Nilanjana Maulik

机构：

[1] University of Connecticut School of Medicine,Molecular Cardiology and Angiogenesis Laboratory, Department of Surgery

[2] University of Debrecen,Department of Pharmacology

[3] University of Connecticut School of Medicine,Translational Genomics Core, Department of Genetics and Developmental Biology

[4] Harvard Medical School,Department of Radiology

[5] Saint Mary’s Hospital,Department of Surgery

来源：

Functional & Integrative Genomics | 2012年 / 12卷

关键词：

Ischemic preconditioning; Nox2; NADPH oxidase; Gene expression; Cardioprotection;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Several potential sources of reactive oxygen species (ROS) in cells exist. One source is NADPH oxidase, which is especially important for superoxide radical production. Nox2 is a primary regulatory subunit of NADPH oxidase. In the present study, we examined the role of ROS and NADPH oxidase in ischemic preconditioning (IP)-mediated cardioprotection by using Nox2−/− mice. Both wild-type (WT) and Nox2−/− mice were subjected to either 30 min of ischemia followed by 2 h of reperfusion (IR) or IP prior to 30 min ischemia and 2 h of reperfusion. Reduction in left ventricular developed pressure (60.1 versus 63 mmHg), dp/dtmax (893 versus 1,027 mmHg/s), and aortic flow (0.9 versus 1.8 ml/min) was observed in Nox2−/−IPIR compared to WTIPIR along with increased infarct size (33% versus 22%) and apoptosis after 120 min of reperfusion. Differentially regulated genes were demonstrated by comparing gene expression in WTIPIR versus Nox2−/− IPIR hearts. Selected differentially regulated genes such as β-catenin, SRPK3, ERDR1, ACIN1, Syntaxin-8, and STC1 were validated by real-time PCR. Taken together, this is the first report identifying important, differentially expressed genes during ischemic preconditioning in Nox2−/− mice by using microarray analysis.

引用

页码：501 / 514

页数：13

共 274 条

[31]

Shah A(2010)Receptor activation of NADPH oxidases Antioxid Redox Signal 13 467-1484

[32]

Shattock M(2008)Oxidative innate immune defenses by Nox/Duox family NADPH oxidases Contrib Microbiol 15 164-988

[33]

Cave AC(2010)Aquaporin-1 expression and angiogenesis in rabbit chronic myocardial ischemia is decreased by acetazolamide Hear Vessel 25 237-H1771

[34]

Brewer AC(1999)Redox signaling: hydrogen peroxide as intracellular messenger Exp Mol Med 31 53-3950

[35]

Narayanapanicker A(2009)Angiopoietin-1 induces Kruppel-like factor 2 expression through a phosphoinositide 3-kinase/AKT-dependent activation of myocyte enhancer factor 2 J Biol Chem 284 5592-3642

[36]

Ray R(2005)Glycogen-synthase kinase3beta/beta-catenin axis promotes angiogenesis through activation of vascular endothelial growth factor signaling in endothelial cells Circ Res 96 308-18126

[37]

Grieve DJ(2008)Heterozygous disruption of Flk-1 receptor leads to myocardial ischaemia reperfusion injury in mice: application of affymetrix gene chip analysis J Cell Mol Med 12 1284-undefined

[38]

Walker S(2008)Adeno-sh-beta-catenin abolishes ischemic preconditioning-mediated cardioprotection by downregulation of its target genes VEGF, Bcl-2, and survivin in ischemic rat myocardium Antioxid Redox Signal 10 1475-undefined

[39]

Shah AM(1990)Amplified RNA synthesized from limited quantities of heterogeneous cDNA Proc Natl Acad Sci U S A 87 1663-undefined

[40]

Chen W(1998)Reactive oxygen species released from mitochondria during brief hypoxia induce preconditioning in cardiomyocytes J Biol Chem 273 18092-undefined

← 1 2 3 4 5 6 7 8 9 10 →