Identification and characterization of the ficellomycin biosynthesis gene cluster from Streptomyces ficellus

被引：0
作者：
Yang Liu
Meng Li
Huiyan Mu
Shuting Song
Ying Zhang
Kun Chen
Xihong He
Haikuan Wang
Yujie Dai
Fuping Lu
Zhongli Yan
Huitu Zhang
机构：
[1] Tianjin University of Science and Technology,Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, College of Bioengineering
[2] Tianjin University of Science and Technology,Industrial Microbiology Laboratory, College of Biotechnology
[3] Tianjin Economic and Technological Development Zone,undefined
来源：
Applied Microbiology and Biotechnology | 2017年 / 101卷
关键词：
Ficellomycin; Antibiotics; Aziridine; Gene cluster;
D O I：
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中图分类号：
学科分类号：
摘要：
Ficellomycin is a peptide-like antibiotic which exhibits potent in vitro activity against Penicillium oxalicum and Staphylococcus aureus, even against strains resistant to most clinically used antibiotics. The gene cluster responsible for ficellomycin biosynthesis was cloned from Streptomyces ficellus and sequenced. It was found to contain 26 ORFs and is located within 30 kb of contiguous DNA. Targeted disruption of the encoding genes revealed that most were involved in the functional section of ficellomycin biosynthesis, such as peptide assembly, regulation, resistance, and biosynthesis of the precursor of ficellomycin 2-[4-guanidyl-1-azabicyclo[3.1.0]hexan-2-yl] glycine (2-GAHG). Within the 2-GAHG synthesis pathway, a sulfate adenylyltransferase appears to be involved in the synthesis of the pharmaceutically important 1-azabicyclo[3.1.0]hexane ring moiety, which has been reported to cause DNA cross-linking or impairment of semiconservative DNA replication.
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页码：7589 / 7602
页数：13
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