Differential expression of TGF-β isoforms during postlactational mammary gland involution

被引：39

作者：

Faure E. ^{[1
]}

Heisterkamp N. ^{[1
]}

Groffen J. ^{[1
]}

Kaartinen V. ^{[2
]}

机构：

[1] Section of Molecular Carcinogenesis, Division of Hematology and Oncology, Childrens Hosp. Los Angeles Res. I., Los Angeles, CA 90027

[2] Developmental Biology Program, Department of Pathology, Mail Stop #54, Los Angeles, CA 90027

来源：

Cell and Tissue Research | 2000年 / 300卷 / 1期

关键词：

Extracellular matrix; Mouse [B6CBA(F[!sub]1[!/sub]); Postlactational involution; Remodeling; TGF-β;

D O I：

10.1007/s004410000183

中图分类号：

学科分类号：

摘要：

After cessation of lactation, the mammary gland undergoes involution, which is characterized by a massive epithelial cell death and proteolytic degradation of the extracellular matrix. Whereas the expression patterns and also the function of TGF-β isoforms during mammary gland branching morphogenesis and lactation are well understood, their expression during postlactational involution and therefore a possible role in this process is poorly known. In this study we show that TGF-β3 expression is dramatically induced (>fivefold) during mouse mammary gland involution when compared to that of virgin mouse, reaching a maximal expression level at day 4 after weaning. In contrast, other TGF-β isoforms do not display significant increase in expression during involution (TGF-β1, 1.3-fold and TGF-β2, <1.5-fold) when compared to that of virgin or lactating mice. During mammary gland involution, TGF-β3 is expressed in the epithelial layer and particularly in myoepithelial cells. A comparison of the kinetics of TGF-β3 expression to that of programmed cell death and degradation of the basement membrane suggests that TGF-β3 functions in the remodeling events of the extracellular matrix during the second stage of involution.

引用

页码：89 / 95

页数：6

共 24 条

[1]

Baik M.G., Lee M.J., Choi Y.J., Gene expression during involution of mammary gland, Int J Mol Med, 2, pp. 39-44, (1998)

[2]

Basbaum C.B., Werb Z., Focalized proteolysis: Spatial and temporal regulation of extracellular matrix degradation at the cell surface, Curr Opin Cell Biol, 8, pp. 731-738, (1996)

[3]

Chomzynski P., Sacchi N., Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction, Anal Biochem, 162, pp. 156-159, (1987)

[4]

Dickson S.R., Warburton M.J., Enhanced synthesis of gelatinase and stromelysin by myoepithelial cells during involution of the rat mammary gland, J Histochem Cytochem, 40, pp. 697-703, (1992)

[5]

Fitzpatrick D.R., Denhez F., Kondaiah P., Akhurst R., Differential expression of TGF-beta isoforms in murine palatogenesis, Development, 109, pp. 585-595, (1990)

[6]

Harlow E., Lane D., Antibodies, A Laboratory Manual, (1988)

[7]

Heldin C.H., Miyazano K., Ten Dijke P., TGF-β signaling from cell membrane to nucleus through SMAD proteins, Nature, 390, pp. 465-471, (1997)

[8]

Jhappan C., Geiser A.G., Kordon E.C., Targeting of a transforming growth factor-β1 transgene to the pregnant mammary gland inhibits alveolar development and lactation, EMBO J, 12, pp. 1835-1845, (1993)

[9]

Kaartinen V., Voncken J.W., Shuler C., Warburton D., Bu D., Groffen J., Abnormal lung development and cleft palate in mice lacking TGF-β3 indicates defects of epithelial-mesenchymal interaction, Nature Genet, 11, pp. 415-421, (1995)

[10]

Kaartinen V., Cui X.-M., Heisterkamp N., Groffen J., Shuler C., Transforming growth factor-β3 regulates transdifferentiation of medial edge epithelium during palatal fusion and associated degradation of the basement membrane, Dev Dyn, 209, pp. 255-260, (1997)

← 1 2 3 →