Interaction of β-Giardin with the Bop1 Protein in Giardia lamblia

被引：0
作者：
J. Kim
S. Y. Goo
H. J. Chung
H. -W. Yang
T. -S. Yong
K. -H. Lee
S. -J. Park
机构：
[1] Yonsei University College of Medicine,Department of Parasitology and Institute of Tropical Medicine, The Brain Korea 21 Project
[2] Hankuk University of Foreign Studies,Department of Environmental Science
[3] Wangsan-Li,undefined
[4] Mohyun-Myun,undefined
来源：
Parasitology Research | 2006年 / 98卷
关键词：
Cell Cycle Control; Ribosome Biogenesis; Yeast Nitrogen Base; WD40 Repeat; Immunoaffinity Column;
D O I：
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学科分类号：
摘要：
A cDNA clone encoding a putative Bop1 homologous protein was identified in Giardia lamblia. Since Bop1 is a nucleolar protein involved in rRNA processing, thereby controlling the cell cycle, we investigated components of cell cycle control in G. lamblia by identifying the protein(s) that interact with Bop 1. Through an immunoaffinity column made with polyclonal antibodies specific to the recombinant Bop1 of G. lamblia, a pool of proteins was obtained from the crude extracts of Giardia and then used as antigens to immunize rats. By employing the resultant sera for cDNA library immunoscreening, we isolated cDNA clones encoding an immunopurified protein, which turned out to contain the gene for β-giardin, a Giardia-specific cytoskeletal protein. The interaction between Bop1 and β-giardin was confirmed via two different methods, yeast two-hybrid assay and coimmunoprecipitation.
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页码：138 / 144
页数：6
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