Structure of the 30S translation initiation complex

被引：0

作者：

Angelita Simonetti

Stefano Marzi

Alexander G. Myasnikov

Attilio Fabbretti

Marat Yusupov

Claudio O. Gualerzi

Bruno P. Klaholz

机构：

[1] Institute of Genetics and of Molecular and Cellular Biology,Department of Structural Biology and Genomics

[2] Inserm,Department of Biology MCA

[3] U596,undefined

[4] Illkirch F-67404,undefined

[5] France ,undefined

[6] CNRS,undefined

[7] UMR7104,undefined

[8] Illkirch F-67404,undefined

[9] France ,undefined

[10] Université Louis Pasteur,undefined

[11] Architecture et Réactivité de l’ARN,undefined

[12] UPR 9002 CNRS,undefined

[13] Institute of Molecular and Cellular Biology,undefined

[14] 15 rue R. Descartes,undefined

[15] 67084 Strasbourg,undefined

[16] France ,undefined

[17] Laboratory of Genetics,undefined

[18] University of Camerino,undefined

来源：

Nature | 2008年 / 455卷

关键词：

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Proteins known as initiation factors (IFs) help to position the 30S subunit of the ribosome correctly on the mRNA start site so that the proper reading frame is engaged. After formation of this 30S initiation complex, the 50S ribosomal subunit binds and the IFs are released. In this work, Simonetti et al. use cryo-electron microscopy to examine the position of the mRNA, initiator tRNA, IF1 and IF2 in the 30S particle. From this structure, they predict how the 50S subunit interacts with the 30S initiation complex, and how GTP bound to IF2 is positioned towards the incoming GTPase-activated centre of the 50S.

引用

页码：416 / 420

页数：4

共 50 条

[31] SUBSPECIES OF E-COLI 30S RIBOSOMES - ELECTROPHORETIC MOBILITY AND ACTIVITY IN INITIATION COMPLEX-FORMATION
HERMOSO, JM
SZER, W
FEDERATION PROCEEDINGS, 1975, 34 (03) : 521 - 521
[32] RIBOSOMAL-PROTEINS DIRECTLY INTERACTING WITH FMET-TRANSFER RNAFMET WITHIN THE 30S INITIATION COMPLEX
BROUDE, NE
MEDVEDEVA, NI
KUSOVA, KS
BUDOVSKII, EI
MOLECULAR BIOLOGY, 1985, 19 (05) : 1040 - 1043
[33] TOPOGRAPHY OF THE ESCHERICHIA-COLI RIBOSOMAL 30S SUBUNIT-INITIATION FACTOR-II COMPLEX
WAKAO, H
ROMBY, P
EBEL, JP
GRUNBERGMANAGO, M
EHRESMANN, C
EHRESMANN, B
BIOCHIMIE, 1991, 73 (7-8) : 991 - 1000
[34] ISOLATION OF PROTEIN INITIATION FACTORS FROM 30S RIBOSOMAL SUBUNITS
PARENTIROSINA, R
EISENSTADT, A
EISENSTADT, JM
NATURE, 1969, 221 (5178) : 363 - +
[35] RIBOSOMAL LOCALIZATION OF THE MESSENGER-RNA IN THE 30S INITIATION COMPLEX AS REVEALED BY UV CROSS-LINKING
BRANDT, R
GUALERZI, CO
FEBS LETTERS, 1992, 311 (03) : 199 - 202
[36] INITIATION COMPLEX-FORMATION ON EUGLENA CHLOROPLAST 30S SUBUNITS IN THE PRESENCE OF NATURAL MESSENGER-RNAS
WANG, CC
RONEY, WB
ALSTON, RL
SPREMULLI, LL
NUCLEIC ACIDS RESEARCH, 1989, 17 (23) : 9735 - 9747
[37] The central pseudoknot in 16S ribosomal RNA is needed for ribosome stability but is not essential for 30S initiation complex formation
Poot, RA
Pleij, CWA
vanDuin, J
NUCLEIC ACIDS RESEARCH, 1996, 24 (19) : 3670 - 3676
[38] Structural Uniqueness and Specific Recognition of Translation Initiation Factor 1 to the 30S Ribosomal Subunit in Pseudomonas Aeruginosa Protein Synthesis
Zhang, Yonghong
Bulllard, James
PROTEIN SCIENCE, 2018, 27 : 35 - 36
[39] Cryo-electron microscopy structure of the 30S subunit in complex with the YjeQ biogenesis factor
Jomaa, Ahmad
Stewart, Geordie
Mears, Jason A.
Kireeva, Inga
Brown, Eric D.
Ortega, Joaquin
RNA, 2011, 17 (11) : 2026 - 2038
[40] THREATENING 30S
DURHAM, GG
PSYCHOLOGY TODAY, 1973, 7 (05) : 16 - 16

← 1 2 3 4 5 →