Surface plasmon resonance detection of silver ions and cysteine using DNA intercalator-based amplification

被引：0
作者：
Chia-Chen Chang
Shenhsiung Lin
Shih-Chung Wei
Yu Chu-Su
Chii-Wann Lin
机构：
[1] National Taiwan University,Institute of Biomedical Engineering
[2] National Yang-Ming University,Department of Medicine
[3] National Taiwan University,Institute of Biomedical Electronic and Bioinformatics
[4] National Taiwan University Hospital,Department of Laboratory Medicine
来源：
Analytical and Bioanalytical Chemistry | 2012年 / 402卷
关键词：
Silver ion; Cysteine; Surface plasmon resonance; DNA intercalator; Cytosine–cytosine mismatch;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
We report the development of a surface plasmon resonance sensor based on the silver ion (Ag+)-induced conformational change of a cytosine-rich, single-stranded DNA for the detection of Ag+ and cysteine (Cys) in aqueous solutions. In the free state, single-stranded oligonucleotides fold into double-helical structures through the addition of Ag+ to cytosine–cytosine (C–C) mismatches. However, in the presence of Cys, which competitively binds to Ag+, the formation of the C–Ag+–C assembly is inhibited, resulting in free-state, single-stranded oligonucleotides. To enhance sensitivity, the DNA intercalator, daunorubicin, was employed to achieve signal enhancement. The detection limit for Ag+ was 10 nM with a measurement range of 50–2,000 nM, and the detection limit for Cys was 50 nM with a measurement range of 50–2,000 nM. This simple assay was also used to individually determine the spiked Ag+ concentration in water samples and Cys concentrations in biological fluid samples.
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页码：2827 / 2835
页数：8
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