Identification of genomic locations associated with grain micronutrients (iron and zinc) in rice (Oryza sativa L.)

被引：0
作者：
Amit Pippal
Nabin Bhusal
Rahul Kumar Meena
Mahavir Bishnoi
Pratik Istari Bhoyar
Rajinder K. Jain
机构：
[1] CCS Haryana Agricultural University,Department of Molecular Biology, Biotechnology and Bioinformatics
[2] CCS Haryana Agricultural University,Department of Genetics and Plant Breeding
[3] Agriculture and Forestry University,Department of Genetics and Plant Breeding
来源：
Genetic Resources and Crop Evolution | 2022年 / 69卷
关键词：
Recombinant inbred lines; Quantitative trait loci; Rice; Micronutrients; Biofortification;
D O I：
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中图分类号：
学科分类号：
摘要：
In this investigation, recombinant inbred lines (RILs) population derived from PAU 201 (high yielding) and Palman 579 (high iron and zinc content) varieties were phenotyped in F5 and F6 generations for grain micronutrient content. The results showed high genetic variations among RILs and exceeded trait values beyond those of parents revealing transgressive segregants. Pearson’s correlation coefficient analysis showed that iron content had a significant positive correlation with zinc content in both generations. Composite interval mapping identified 16 QTLs on linkage groups 2, 6, 10, and 12. Eight QTLs were for zinc content and four for iron content, and four for grain yield per plant. The maximum number of QTLs were detected on chromosome 2, followed by chromosomes 12 and 10. The LOD score of identified QTLs varied from 3.7 (qYp10) to 18.43 (qFe2) explaining 51.8% and 52.8% phenotypic variation, respectively. Co-localization of QTLs (qZn12.2, qFe12, and qYp12) associated with zinc content, iron content, and grain yield in 12 between the marker interval RM 2734 and RM12 represents 5.1 cM distance, could be used for introgression for rice improvement through marker-assisted selection after validation.
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页码：221 / 230
页数：9
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