Chemical shift assignments of a calmodulin intermediate with two Ca2+ bound in complex with the IQ-motif of voltage-gated Ca2+ channels (CaV1.2)

被引：0
作者：
Ian Salveson
David E. Anderson
Johannes W. Hell
James B. Ames
机构：
[1] University of California,Department of Chemistry
[2] University of California,Department of Pharmacology
来源：
Biomolecular NMR Assignments | 2019年 / 13卷
关键词：
Calmodulin; EF-hand; Ca; 1.2; IQ-motif; Synaptic plasticity; CDI;
D O I：
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摘要：
Calcium-dependent inactivation (CDI) of neuronal voltage-gated Ca2+ channels (CaV1.2) is important for synaptic plasticity, which is associated with learning and memory. The Ca2+-dependent binding of calmodulin (CaM) to CaV1.2 is essential for CDI. Here we report NMR assignments for a CaM mutant (D21A/D23A/D25A/E32Q/D57A/D59A/N61A/E68Q, called CaMEF12) that contains two Ca2+ bound at the third and fourth EF-hands (EF3 and EF4) and is bound to the IQ-motif (residues 1644–1665) from CaV1.2 (BMRB accession no. 27692).
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页码：233 / 237
页数：4
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共 121 条
  • [1] Adams PJ(2014)Apocalmodulin itself promotes ion channel opening and Ca(2+) regulation Cell 159 608-622
  • [2] Ben-Johny M(2013)Dynamic switching of calmodulin interactions underlies Ca Nat Commun 4 1717-293
  • [3] Dick IE(1995) regulation of CaV1.3 channels J Biomol NMR 6 277-985
  • [4] Inoue T(2001)NMRPipe: a multidimensional spectral processing system based on UNIX pipes Neuron 31 973-3234
  • [5] Yue DT(2013)Preassociation of calmodulin with voltage-gated Ca(2+) channels revealed by FRET in single living cells J Mol Biol 425 3217-836
  • [6] Ben Johny M(2014)Apo states of calmodulin and CaBP1 control CaV1 voltage-gated calcium channel function through direct competition for the IQ domain Nat Commun 5 5126-962
  • [7] Yang PS(1982)Regulation of the NaV1.5 cytoplasmic domain by calmodulin Biochem Biophys Res Commun 104 830-4667
  • [8] Bazzazi H(1993)Ca J Cell Biol 123 949-9228
  • [9] Yue DT(1990)-induced hydrophobic site on calmodulin: application for purification of calmodulin by phenyl-Sepharose affinity chromatography Biochemistry 29 4659-1327
  • [10] Delaglio F(1991)Identification and differential subcellular localization of the neuronal class C and class D L-type calcium channel alpha 1 subunits Biochemistry 30 9216-9892
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