Electrochemical gene sensor based on a glassy carbon electrode modified with hemin-functionalized reduced graphene oxide and gold nanoparticle-immobilized probe DNA

被引：0
作者：
Yongkang Ye
Jiaona Gao
Hao Zhuang
Haisong Zheng
Hanju Sun
Yingwang Ye
Xuan Xu
Xiaodong Cao
机构：
[1] Hefei University of Technology,College of Food Science and Engineering
[2] University of Siegen,Institute of Materials Engineering
[3] Technology Center of Anhui Entry-Exit Inspection and Quarantine Bureau,College of Chemistry and Molecular Engineering
[4] Nanjing Tech University,undefined
来源：
Microchimica Acta | 2017年 / 184卷
关键词：
Electrochemical biosensor; Hemin-rGO conjugate; Bioconjugation; Electrochemical deposition; Field emission transmission electron microscopy; X-ray photoelectron spectroscopy; Ultraviolet-visible spectroscopy; Cyclic voltammetry; Differential pulse voltammetry;
D O I：
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中图分类号：
学科分类号：
摘要：
The authors describe an electrochemical DNA biosensor based on a glassy carbon electrode modified with gold nanoparticles (AuNPs) and reduced graphene oxide that was functionalized with hemin (hemin-rGO). Capture DNA (a 21-mer) is immobilized on the AuNPs via gold-thiol chemistry. The detection scheme relies on the measurement of the differential pulse voltammetric current change of the oxidation-reduction reaction of hemin before and after DNA hybridization has occurred. The decrease in the voltammetric currents of iron (III) (best measured at −0.3 V vs. Ag/AgCl) is linearly related to the concentration of complementary DNA. The calibration plot covers the 1.0 aM to 0.1 pM concentration range, and the detection limit is as low as 0.14 aM (at a signal-to-noise ratio of 3). The biosensor was successfully applied to the direct determination of complementary DNA in self-prepared samples by using the standard addition method.
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页码：245 / 252
页数：7
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