Identification of citrus hybrids through the combination of leaf apex morphology and SSR markers

被引：54
作者：
De Oliveira A.C. [1 ]
Novac Garcia A. [1 ]
Cristofani M. [1 ]
Machado M.A. [1 ]
机构：
[1] Citriculture Center ‘Sylvio Moreira’ of Agronomic Institute, Cordeiropolis, Sao Paulo 13490-970
来源：
Euphytica | 2002年 / 128卷 / 3期
基金：
巴西圣保罗研究基金会;
关键词：
Apomixis; Breeding; Citrus; Hybrid; Microsatellite; Seedling selection;
D O I：
10.1023/A:1021223309212
中图分类号：
学科分类号：
摘要：
Citrus breeding programs normally face several biological obstacles such as apomixis and polyembryony that result in a cumbersome identification of hybrid seedlings. The main purpose of this work is to describe the combined use of visual selection based on the leaf apex morphology and SSR analysis to differentiate hybrid from nucellar seedlings derived from the cross between the 'Murcott' tangor [Citrus reticulata Blanco x Citrus sinensis (L.) Osb.] and 'Pêra' sweet orange [Citrus sinensis (L.) Osb.]. A new morphological variable named leaf apex morphometric index is also described as the quantitative basis of the visual selection. The efficiency of visual selection of hybrids was tested under two growth conditions, seedlings germinated in seedbeds and in plastic tubes. Putative hybrid seedlings were also confirmed through the analysis of simple sequence repeats (SSR). The visual selection of hybrid seedlings resulted in an increase of 87.2% (p < 0.01) and 202.2% (p < 0.001) in the number of correctly identified hybrids when compared to the method of random picking of seedlings in seedbeds and plastic tubes, respectively. The results indicate that the combination of visual selection and SSR analysis for the identification of hybrids derived from the cross of polyembryonic citrus cultivars will improve the accuracy of the selection, save time, and reduce the costs involved in the use of molecular markers alone in citrus breeding programs.
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页码：397 / 403
页数：6
相关论文
共 26 条
  • [1] Anderson C.M., Castle W.S., Moore G.A., Isozymic identification of zygotic seedlings in Swingle citrumelo Citrus paradisi x Poncirus trifoliata nursery and field populations, J Amer Soc Hort Sci, 116, pp. 322-326, (1991)
  • [2] Ballve R.M.L., Medina Filho H.P., Bordignon R., Identification of reciprocal hybrids in citrus by the broadness of the leaf petiole wing, Braz J Genet, 20, pp. 697-702, (1997)
  • [3] Bastianel M., Schwarz S.F., Colleta Filho H.D., Lin L.L., Machado M.A., Koller O.C., Identification of zygotic and nucellar tangerine seedlings (Citrus spp.) using RAPD, Genet Mol Biol, 21, pp. 123-127, (1998)
  • [4] Bercovich D., Regev Z., Ratz T., Luder A., Plutsky Y., Gruenbaum Y., Quantitative ratio of primer pair and annealing temperature affecting PCR products in duplex amplification, Biotechniques, 27, pp. 762-770, (1999)
  • [5] Cameron J.W., Frost H.B., Genetics, breeding, and nucellar embryony, The Citrus Industry, 2, pp. 325-370, (1968)
  • [6] Cooper W.C., Reece P.C., Furr J.R., Citrus breeding in Florida: Past, present and future, Proc Florida State Hort Soc, 75, pp. 5-13, (1962)
  • [7] Cristofani M., Brondani R.V., Novelli V.M., Machado M.A., Desenvolvimento de marcadores microsatélites para análise genética em citros, Genet Mol Biol, 21, (1998)
  • [8] Domingues E.T., Caracterização Morfológica, Agronômica, Isoenzimática e por RAPD de Variedades de Laranja Doce - Citrus sinensis (L.) Osbeck, (1998)
  • [9] Donadio L.C., Estudo de Alguns Caracteres Morfológicos de Progênies Obtidas de Hibridações de Cultivares Poliembriônicas de Citrus, (1979)
  • [10] Frost H.B., Soost R.K., Seed reproduction: Development of gametes and embryos, The Citrus Industry, 2, pp. 290-324, (1968)
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