Stimulated Raman scattering microscopy with a robust fibre laser source

被引：0

作者：

Freudiger C.W. ^{[1
,2
]}

Yang W. ^{[2
]}

Holtom G.R. ^{[2
]}

Peyghambarian N. ^{[3
]}

Xie X.S. ^{[2
]}

Kieu K.Q. ^{[3
]}

机构：

[1] Invenio Imaging, Inc., Menlo Park, CA 94025

[2] Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138

[3] College of Optical Science, University of Arizona, Tucson, AR 85721

来源：

Nature Photonics | 2014年 / 8卷 / 2期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

D O I：

10.1038/nphoton.2013.360

中图分类号：

学科分类号：

摘要：

Stimulated Raman scattering microscopy allows label-free chemical imaging and has enabled exciting applications in biology, material science and medicine. It provides a major advantage in imaging speed over spontaneous Raman scattering and has improved image contrast and spectral fidelity compared to coherent anti-Stokes Raman scattering. Wider adoption of the technique has, however, been hindered by the need for a costly and environmentally sensitive tunable ultrafast dual-wavelength source. We present the development of an optimized all-fibre laser system based on the optical synchronization of two picosecond power amplifiers. To circumvent the high-frequency laser noise intrinsic to amplified fibre lasers, we have further developed a high-speed noise cancellation system based on voltage-subtraction autobalanced detection. We demonstrate uncompromised imaging performance of our fibre-laser-based stimulated Raman scattering microscope with shot-noise-limited sensitivity and an imaging speed up to 1 frame s -1. © 2014 Macmillan Publishers Limited.

引用

页码：153 / 159

页数：6

共 35 条

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