mRNA display for the selection and evolution of enzymes from in vitro-translated protein libraries

被引：0
作者：
Burckhard Seelig
机构：
[1] Molecular Biology and Biophysics,Department of Biochemistry
[2] BioTechnology Institute,undefined
[3] University of Minnesota,undefined
来源：
Nature Protocols | 2011年 / 6卷
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摘要：
The mRNA display technology enables the in vitro selection and directed evolution of functional proteins from libraries of more than 1012 different mutants in a single test tube. The size of these libraries is well beyond the limit of screening technologies and of most in vivo and in vitro selection methods. The mRNA display technology has been used to select peptides and proteins that bind to a specific ligand, as well as novel enzymes. This protocol details the procedure to produce mRNA-displayed proteins (3 d) and to subject them to a selection and evolution of enzymes for bond-forming reactions (4–10 weeks). This method is demonstrated by the generation of new RNA ligase enzymes.
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页码：540 / 552
页数：12
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