Enhanced production of penicillin G acylase from a recombinant Escherichia coli

被引：0
|
作者：
Pawan Kumar Vohra
Rakesh Sharma
Des Raj Kashyap
Rupinder Tewari
机构：
[1] Panjab University,Department of Microbiology
[2] Institute of Microbial Technology,Biochemical Engineering and Process Development Centre
[3] Panjab University,Department of Biotechnology
来源：
Biotechnology Letters | 2001年 / 23卷
关键词：
penicillin G acylase gene; plasmid stability;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Penicillin G acylase (pac) gene was cloned into a stable asd+ vector (pYA292) and expressed in Escherichia coli. This recombinant strain produced 1000 units penicillin G acylase g−1 cell dry wt, which is 23-fold more than that produced by parental Escherichia coli ATCC11105. This enzyme was purified to 16 units mg−1 protein by a novel two-step process.
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页码：531 / 535
页数：4
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