Extracellular production of lipoxygenase from Anabaena sp. PCC 7120 in Bacillus subtilis and its effect on wheat protein

被引：0
作者：
Chong Zhang
Tingting Tao
Qi Ying
Dongliang Zhang
Fengxia Lu
Xiaomei Bie
Zhaoxin Lu
机构：
[1] Nanjing Agriculture University,Laboratory of Enzyme Engineering, College of Food Science and Technology
来源：
Applied Microbiology and Biotechnology | 2012年 / 94卷
关键词：
sp. PCC 7120; Recombinant lipoxygenase (ana-rLOX); Dough protein;
D O I：
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学科分类号：
摘要：
In this study, the lipoxygenase (ana-LOX) gene from Anabaena sp. PCC 7120 was successful expressed and secreted in Bacillus subtilis. Under the control of the P43 promoter, with a signal peptide from the B. subtilis 168 nprB gene, and facilitated by the molecular chaperone PrsA, the production of the recombinant ana-LOX (ana-rLOX) reached 76 U/mL (171.9 μg/ml) in the supernatant. The purified ana-rLOX was investigated for its effect on dough protein. Ana-rLOX treatment decreased free sulfhydryl groups, increased glutenin macropolymer content, promoted the formation of covalent bonds between gluten protein, and affected protein crosslinking. The results indicated that large aggregates involving gliadin and glutenin were formed. The glutenin macropolymer played a role in the formation of the dough network structure through the exchange of thiol disulfide bonds and the formation of hydrogen or hydrophobic bonds with other proteins.
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页码：949 / 958
页数：9
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  • [1] Anderson AK(2000)Changes in disulfide and sulfhydryl contents and electrophoretic patterns of extruded wheat flour proteins Cereal Chem 77 354-359
  • [2] Andreou AZ(2008)Properties of a mini 9R-lipoxygenase from Phytochemistry 9 1832-1837
  • [3] Vanko M(2007) sp. PCC 7120 and its mutant forms Enzyme Microb Technol 40 491-496
  • [4] Bezakova L(1998)Lipoxygenase in fruits and vegetables: A review J Biol Chem 273 23225-23232
  • [5] Feussner I(1999)The rabbit 15-lipoxygenase perferentially oxygenates LDL cholesterol esters, and this reaction does not require vitamin E J Biol Chem 274 23679-23682
  • [6] Baysal Y(2006)Lipoxygenases: occurrence, functions, catalysis, and acquisition of substrate J Mol Biol 362 393-402
  • [7] Demirdöven A(1998)Systematic screening of all signal peptides from J Biotechnol 64 3-13
  • [8] Belkner J(1999): a powerful strategy in optimizing heterologous protein secretion in Gram-positive bacteria Trends Food Sci. Tech 10 297-302
  • [9] Stender H(1987)Protein secretion and possible roles for multiple signal peptidases for precursor processing in Bacilli Methods Enzymol 153 507-516
  • [10] Kühn H(2004)New frontiers in food enzymology: recombinant lipoxygenases Appl Environ Microbiol 70 5704-5707
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