Callus induction and somatic embryogenesis of Phalaenopsis

被引：0

作者：

Y. Ishii

T. Takamura

M. Goi

M. Tanaka

机构：

[1] Department of Horticulture,

[2] Faculty of Agriculture,undefined

[3] Kagawa University,undefined

[4] Miki-cho,undefined

[5] Kagawa 761-0795,undefined

[6] Japan,undefined

来源：

Plant Cell Reports | 1998年 / 17卷

关键词：

Key wordsPhalaenopsis; Protocorm-like bodies; Callus induction; Somatic embryogenesis;

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学科分类号：

摘要：

Callus induction and plant regeneration through somatic embryogenesis in Phalaenopsis Richard Shaffer `Santa Cruz' were examined. Protocorm-like body (PLB) segments formed calli in Vacin and Went medium with sucrose. The optimal concentration of sucrose was 40 g ⋅ l–1. Medium containing 200 ml ⋅ l–1 coconut water together with 40 g ⋅ l–1 sucrose was effective for callus induction. Gellan gum was suitable than agar as a gelling agent for callus induction. The calli easily formed PLBs after being transferred to a medium without sucrose. Histological observation suggested that the PLBs were somatic embryos. No variation was observed in the flowering plants regenerated through somatic embryogenesis.

引用

页码：446 / 450

页数：4