Isolation and characterization of a novel cDNA encoding methyl jasmonate-responsive transcription factor TcAP2 from Taxus cuspidata

被引：0
作者：
Yiling Dai
Qiulin Qin
Dalin Dai
Lisheng Kong
Wei Li
Xiaojun Zha
Yuanjie Jin
Kexuan Tang
机构：
[1] Fudan University,State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan
[2] Southwest China University,SJTU
[3] University of Victoria,Nottingham Plant Biotechnology R&D Center
来源：
Biotechnology Letters | 2009年 / 31卷
关键词：
AP2/EREBP domain; Methyl jasmonate; Transcription factor; Yeast one-hybrid;
D O I：
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中图分类号：
学科分类号：
摘要：
A novel transcription factor, TcAP2, was isolated from Taxus cuspidata by yeast one-hybrid strategy. This factor interacts with jasmonate- and elicitor-responsive element. Analysis of the deduced TcAP2 amino acid sequence revealed that TcAP2 contained a conserved AP2/ethylene-responsive element binding protein domain that consisted of 268 amino acids in a potential nuclear localization sequence. The factor of TcAP2 had a high homology, in its AP2 domain, to other AP2 family members. Based on phylogenetic analysis, it was different from other five DRE-binding proteins in their evolutionary relationship. The transcription of TcAP2 gene in yew accumulated primarily in young organs, such as young stems. Quantitative real-time RT-PCR analysis indicated that TcAP2 gene was inducible to express by treatments with methyl jasmonate plus salicylic acid, high salinity, and cold. This gene showed no response to either abscisic acid or drought treatment.
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页码：1801 / 1809
页数：8
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