Mechanical Interactions of Mouse Mammary Gland Cells with Collagen in a Three-Dimensional Construct

被引：0
作者：
M. d. C. Lopez-Garcia
D. J. Beebe
W. C. Crone
机构：
[1] University of Wisconsin,Material Science Program, Wisconsin Institutes for Medical Research
[2] University of Wisconsin,Department of Biomedical Engineering, Wisconsin Institutes for Medical Research
[3] University of Wisconsin – Madison,Department of Engineering Physics
来源：
Annals of Biomedical Engineering | 2010年 / 38卷
关键词：
Stress; Strain; Collagen; Young’s modulus; Mammographic density;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
An effort to understand the development of breast cancer motivates the study of mammary gland cells and their interactions with the extracellular matrix. A mixture of mammary gland epithelial cells (normal murine mammary gland), collagen, and fluorescent beads was loaded into microchannels and observed via four-dimensional imaging. Collagen concentrations of 1.3, 2, and 3 mg/mL were used. The displacements of the beads were used to calculate strains in the 3D matrix. To ensure physiologically relevant materials properties for analysis, the collagen was characterized using independent tensile testing with strain rates in the range of those measured in the cell–gel constructs. 3D elastic theory for an isotropic material was employed to calculate the stress. The technique presented adds to the field of measuring cell-generated stresses by providing the capability of measuring 3D stresses locally around a single cell and using physiologically relevant materials properties for analysis. The highest strains were observed in the most compliant matrix. Additionally, the stresses fluctuated over time due to the cells’ interaction with the collagen matrix.
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页码：2485 / 2498
页数：13
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