Resolution of salbutamol enantiomers in human urine by reversed-phase high performance liquid chromatography after derivatization with (S)-(-)-α-methylbenzyl isocyanate

被引：0
作者：
Kyeong Ho Kim
Tae Kyun Kim
Young Hee Kwon
Young Taek Sohn
机构：
[1] Kangwon National University,College of Pharmacy
[2] Choseon Pharm. & Trading Co.,Institute of Life Science Research
[3] Seoul National University Hospital,Department of Pharmacy
[4] Duksung Women's University,College of Pharmacy
来源：
Archives of Pharmacal Research | 1997年 / 20卷
关键词：
Chiral separation; HPLC; Chiral derivatizing reagent; Salbutamol; Enantiomer; (S)-(−)-α-methylbenzyl isocyanate;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A stereospecific HPLC method has been developed for the resolution of the enantiomers of salbutamol in human urine. After solid-phase extraction and derivatization with (S)-(-)-α-methylbenzyl isocyanate, the diastereomeric derivatives were resolved (Rs=1.59) on 5 μm octadecylsilan column using 47% methanol as a mobile phase with fluorescence detection. The detection limit of each enantiomer was 10 ng/ml (S/N=3).
引用
收藏
页码：486 / 490
页数：4
相关论文
共 50 条
  • [41] Comment on: Development and validation of a reversed-phase high-performance liquid chromatography assay for polymyxin B in human plasma
    Tam, Vincent H.
    Hou, JingGuo
    Kwa, Andrea L.
    Prince, Randall A.
    JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2009, 63 (03) : 627 - 628
  • [42] Fractionation of Dissolved Organic Matter on Coupled Reversed-Phase Monolithic Columns and Characterisation Using Reversed-Phase Liquid Chromatography-High Resolution Mass Spectrometry
    Sandron, Sara
    Davies, Noel W.
    Wilson, Richard
    Cardona, Alfonso Rojas
    Haddad, Paul R.
    Nesterenko, Pavel N.
    Paull, Brett
    CHROMATOGRAPHIA, 2018, 81 (02) : 203 - 213
  • [43] Influence of phase type and solute structure on changes in retention with pressure in reversed-phase high performance liquid chromatography
    Fallas, Morgane M.
    Tanaka, Nobuo
    Buckenmaier, Stephan M. C.
    McCalley, David V.
    JOURNAL OF CHROMATOGRAPHY A, 2013, 1297 : 37 - 45
  • [44] Determination of 8 Synthetic Food Dyes by Solid Phase Extraction and Reversed-Phase High Performance Liquid Chromatography
    Mazdeh, Fatemeh Zamani
    Khorrami, Afshin Rajabi
    Moradi-Khatoonabadi, Zhila
    EsmaeiliAftabdari, Farideh
    Ardekani, Mohammad Reza Shams
    Moghaddam, Ghazaleh
    Hajimahmoodi, Mannan
    TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH, 2016, 15 (01) : 173 - 181
  • [45] Study of overload for basic compounds in reversed-phase high performance liquid chromatography as a function of mobile phase pH
    Davies, Nicola H.
    Euerby, Melvin R.
    McCalley, David V.
    JOURNAL OF CHROMATOGRAPHY A, 2006, 1119 (1-2) : 11 - 19
  • [46] Determination of pineal melatonin by precolumn derivatization reversed-phase high-performance liquid chromatography and its application to the study of circadian rhythm in rats and mice
    Hamase, K
    Tomita, T
    Kiyomizu, A
    Zaitsu, K
    ANALYTICAL BIOCHEMISTRY, 2000, 279 (01) : 106 - 110
  • [47] Simultaneous Determination of Amoxicillin and Ampicillin in Eggs by Reversed-Phase High-Performance Liquid Chromatography with Fluorescence Detection using Pre-Column Derivatization
    Xie, Kaizhou
    Jia, Longfei
    Xu, Dong
    Guo, Huisheng
    Xie, Xing
    Huang, Yuping
    Chen, Xuesen
    Bao, Wenbin
    Dai, Guojun
    Wang, Jinyu
    JOURNAL OF CHROMATOGRAPHIC SCIENCE, 2012, 50 (07) : 620 - 624
  • [48] Determination of environmental estrogens in human urine by high performance liquid chromatography after fluorescent derivatization with p-nitrobenzoyl chloride
    Mao, LH
    Sun, CJ
    Zhang, H
    Li, YX
    Wu, DS
    ANALYTICA CHIMICA ACTA, 2004, 522 (02) : 241 - 246
  • [49] DETERMINATION OF S-12024 ENANTIOMERS IN HUMAN PLASMA BY LIQUID-CHROMATOGRAPHY AFTER CHIRAL PRECOLUMN DERIVATIZATION
    BOURSIERNEYRET, C
    BAUNE, A
    KLIPPERT, P
    CASTAGNE, I
    SAUVEUR, C
    JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 1993, 11 (11-12) : 1161 - 1166
  • [50] Direct chiral separation of troglitazone stereoisomers using reversed-phase high-performance liquid chromatography
    Suzuki, N
    Takemura, A
    Miyamoto, A
    Yoshioka, T
    Tsutsumi, S
    Kawasaki, T
    JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2002, 30 (03) : 823 - 836
12345