Resolution of salbutamol enantiomers in human urine by reversed-phase high performance liquid chromatography after derivatization with (S)-(-)-α-methylbenzyl isocyanate

被引：0
作者：
Kyeong Ho Kim
Tae Kyun Kim
Young Hee Kwon
Young Taek Sohn
机构：
[1] Kangwon National University,College of Pharmacy
[2] Choseon Pharm. & Trading Co.,Institute of Life Science Research
[3] Seoul National University Hospital,Department of Pharmacy
[4] Duksung Women's University,College of Pharmacy
来源：
Archives of Pharmacal Research | 1997年 / 20卷
关键词：
Chiral separation; HPLC; Chiral derivatizing reagent; Salbutamol; Enantiomer; (S)-(−)-α-methylbenzyl isocyanate;
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摘要：
A stereospecific HPLC method has been developed for the resolution of the enantiomers of salbutamol in human urine. After solid-phase extraction and derivatization with (S)-(-)-α-methylbenzyl isocyanate, the diastereomeric derivatives were resolved (Rs=1.59) on 5 μm octadecylsilan column using 47% methanol as a mobile phase with fluorescence detection. The detection limit of each enantiomer was 10 ng/ml (S/N=3).
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页码：486 / 490
页数：4
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