Cloning of levansucrase from Leuconostoc mesenteroides and its expression in Pichia pastoris

被引：0

作者：

Hee Kyoung Kang

Sang Il Yun

Tae Yoon Lim

Yong-Mei Xia

Doman Kim

机构：

[1] Chonnam National University,The Research Institute for Catalysis

[2] Chonnam National University,Interdisciplinary Program of Graduate School for Bioenergy and Biomaterials

[3] Chonnam National University,School of Biological Sciences and Technology

[4] Korea Advanced Institute of Science and Technology (KAIST),Deptartment of Biological Science

[5] Jiangnan University,State Key Laboratory of Food Science and Technology

来源：

Food Science and Biotechnology | 2011年 / 20卷

关键词：

levansucrase; expression; levan;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

A gene (m1ft) encoding levansucrase from Leuconostoc mesenteroides has been previously cloned in Escherichia coli. Presently, m1ft was cloned and secretively expressed in Pichia pastoris. Methanol induction of recombinant M1FT resulted in the production of active levansucrase (PM1FT). PM1FT-5 was expressed as an active form, but the protein accumulated mainly inside the cells, representing about 5% of total cell proteins. M1FT was secreted into the culture supernatant with a maximum yield of 14,400 U/L using fed-batch fermentations. P. pastoris-derived M1FT displayed catalytic activities comparable to those of the E. coli-derived M1FT. PM1FT was glycosylated at its 2 potential N-glycosylation sites.

引用

页码：277 / 281

页数：4

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