Cloning of levansucrase from Leuconostoc mesenteroides and its expression in Pichia pastoris

被引：0

作者：

Hee Kyoung Kang

Sang Il Yun

Tae Yoon Lim

Yong-Mei Xia

Doman Kim

机构：

[1] Chonnam National University,The Research Institute for Catalysis

[2] Chonnam National University,Interdisciplinary Program of Graduate School for Bioenergy and Biomaterials

[3] Chonnam National University,School of Biological Sciences and Technology

[4] Korea Advanced Institute of Science and Technology (KAIST),Deptartment of Biological Science

[5] Jiangnan University,State Key Laboratory of Food Science and Technology

来源：

Food Science and Biotechnology | 2011年 / 20卷

关键词：

levansucrase; expression; levan;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

A gene (m1ft) encoding levansucrase from Leuconostoc mesenteroides has been previously cloned in Escherichia coli. Presently, m1ft was cloned and secretively expressed in Pichia pastoris. Methanol induction of recombinant M1FT resulted in the production of active levansucrase (PM1FT). PM1FT-5 was expressed as an active form, but the protein accumulated mainly inside the cells, representing about 5% of total cell proteins. M1FT was secreted into the culture supernatant with a maximum yield of 14,400 U/L using fed-batch fermentations. P. pastoris-derived M1FT displayed catalytic activities comparable to those of the E. coli-derived M1FT. PM1FT was glycosylated at its 2 potential N-glycosylation sites.

引用

页码：277 / 281

页数：4

共 50 条

[21] Cloning, expression, and characterization of Aureobasidium melanogenum lipase in Pichia pastoris
Wongwatanapaiboon, Jinaporn
Klinbunga, Sirawut
Ruangchainikom, Chalermchai
Thummadetsak, Gamgarn
Chulalaksananukul, Suphang
Marty, Alain
Chulalaksananukul, Warawut
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2016, 80 (11) : 2231 - 2240
[22] Cloning and Expression of Recombinant Human Thymosin in Yeast Pichia pastoris
曹俊霞
High Technology Letters, 2003, (03) : 60 - 64
[23] Gene cloning and expression of MAP30 in Pichia pastoris
Wang, Fang
Chi, Chun-yu
Wang, Li-yuan
Qiao, Yu
Jin, Xiao-xia
Ding, Guo-hua
BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, 2014, 28 (01) : 136 - 139
[24] Identification and functional characterization of levS, a gene encoding for a levansucrase from Leuconostoc mesenteroides NRRL B-512F
Morales-Arrieta, Sandra
Rodriguez, Maria Elena
Segovia, Lorenzo
Lopez-Munguia, Agustin
Olvera-Carranza, Clarita
GENE, 2006, 376 (01) : 59 - 67
[25] Cloning of a gene encoding thermostable cellobiohydrolase from the thermophilic fungus Chaetomium thermophilum and its expression in Pichia pastoris
Li, Y. -L.
Li, H.
Li, A. -N.
Li, D. -C.
JOURNAL OF APPLIED MICROBIOLOGY, 2009, 106 (06) : 1867 - 1875
[26] Cloning of a gene encoding glycosyltransferase from Pueraria lobata (Wild.) Ohwi and its expression in Pichia pastoris
Zhou, Wenling
Wang, Yinghua
Chen, Gang
Lue, Min
LixiaYang
Hu, Xiaoxiang
Li, Haihang
Li, Ling
AFRICAN JOURNAL OF BIOTECHNOLOGY, 2011, 10 (01): : 85 - 96
[27] CLONING, EXPRESSION AND CHARACTERIZATION OF XYLANASE GENE XYNB FROM ASPERGILLUS NIGER IN PICHIA PASTORIS
Li Fei
Zhao Linguo
Li Guoqing
Yang Shiyi
Li Xun
Wang Fei
RESEARCH PROGRESS IN PAPER INDUSTRY AND BIOREFINERY (4TH ISETPP), VOLS 1-3, 2010, : 637 - 640
[28] Cloning and expression of the XPR2 gene from Yarrowia lipolytica in Pichia pastoris
Poza, M.
Sestelo, A. B. F.
Ageitos, J. M.
Vallejo, J. A.
Veiga-Crespo, P.
Villa, T. G.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2007, 55 (10) : 3944 - 3948
[29] Rhizopus chinensis lipase: Gene cloning, expression in Pichia pastoris and properties
Yu, Xiao-Wei
Wang, Le-Le
Xu, Yan
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 2009, 57 (1-4) : 304 - 311
[30] Molecular cloning and expression of grass carp MyoD in yeast Pichia pastoris
Wang, Lixin
Bai, Junjie
Luo, Jianren
Chen, Hong
Ye, Xing
Jian, Qing
Lao, Haihua
JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2007, 40 (01): : 22 - 28

← 1 2 3 4 5 →