Dynamics of exocyclic groups in the Escherichia coli O91 O-antigen polysaccharide in solution studied by carbon-13 NMR relaxation

被引:0
作者
Maria Soltesova
Jozef Kowalewski
Göran Widmalm
机构
[1] Stockholm University,Arrhenius Laboratory, Department of Materials and Environmental Chemistry
[2] Charles University in Prague,Department of Low Temperature Physics, Faculty of Mathematics and Physics
[3] Stockholm University,Arrhenius Laboratory, Department of Organic Chemistry
来源
Journal of Biomolecular NMR | 2013年 / 57卷
关键词
Carbohydrates; Polysaccharide; Biopolymer; NMR spectroscopy; Model free approach; Two-site jump model;
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摘要
Carbon-13 relaxation data are reported for exocyclic groups of hexopyranosyl sugar residues in the repeating unit within the Escherichia coli O91 O-antigen polysaccharide in a dilute D2O solution. The measurements of T1, T2 and heteronuclear nuclear Overhauser enhancements were carried out at 310 K at two magnetic fields (16.4 T, 21.1 T). The data were analyzed using the standard and extended Lipari–Szabo models, as well as a conformational jump model. The extended version of the Lipari–Szabo and the two-site jump models were most successful for the hydroxymethyl groups of Gal and GlcNAc sugar residues. Different dynamics was found for the hydroxymethyl groups associated with different configurations (d-gluco, d-galacto) of the sugar residues, the latter being faster than the former.
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页码:37 / 45
页数:8
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