Laminin γ1 chain peptide, C-16 (KAFDITYVRLKF), promotes migration, MMP-9 secretion, and pulmonary metastasis of B16-F10 mouse melanoma cells

被引：0

作者：

Kuratomi Y. ^{[1
,2
]}

Nomizu M. ^{[1
,3
]}

Tanaka K. ^{[1
,4
]}

Ponce M.L. ^{[1
]}

Komiyama S. ^{[2
]}

Kleinman H.K. ^{[1
]}

Yamada Y. ^{[1
]}

机构：

[1] Craniofacial Developmental Biology and Regeneration Branch, National Institute of Dental and Craniofacial Research, Maryland, MD 20892, NIH, Bethesda

[2] Department of Otorhinolaryngology, Faculty of Medicine, Kyushu University, Fukuoka 812-8582, 3-1-1 Maidashi, Higashi-ku

[3] Graduate School of Environmental Earth Science, Hokkaido University

[4] Department of Orthopedics, Faculty of Medicine, Kyushu University, Fukuoka 812-8582, 3-1-1 Maidashi, Higashi-ku

来源：

British Journal of Cancer | 2002年 / 86卷 / 7期

关键词：

Adhesion; Laminin; Metastasis; Migration; Peptide;

D O I：

10.1038/sj.bjc.6600187

中图分类号：

学科分类号：

摘要：

Laminin-1, a heterotrimer of α1, β1, and γ1 chains specific to basement membrane, promotes cell adhesion and migration, proteinase secretion and metastases of tumour cells. Several active sites on the α1 chain have been found to promote B16-F10 melanoma lung colonisation and here we have determined whether additional tumour promoting sites exist on the β1 and γ1 chains. Recently, we have identified novel cell adhesive peptides derived from laminin β1 and γ1 chains by systematic screening of synthetic peptides. Nine β1 peptides and seven γ1 peptides active for cell adhesion were tested for their effects on experimental pulmonary metastases of B16-F10 mouse melanoma cells in vivo. The most active adhesive peptide derived from the γ1 chain globular domain, C-16 (KAFDITYVRLKF), significantly enhanced pulmonary metastases of B16-F10 cells, whereas no other peptides showed enhancement. C-16 also stimulated migration of B16-F10 cells in the Boyden chamber assay in vitro. Furthermore, C-16 significantly induced the production of MMP-9 from B16-F10 cells. These results suggest that this specific laminin γ1 chain peptide has a metastasis-promoting activity and might be a new molecular target of anti-cancer treatment. © 2002 Cancer Research UK.

引用

页码：1169 / 1173

页数：4

共 43 条

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