Kinetic model of lysozyme renaturation with the molecular chaperone GroEL

被引：0

作者：

Xiao-Yan Dong

Yu-Bing Wang

Xiao-Guang Liu

Yan Sun

机构：

[1] Tianjin University,Department of Biochemical Engineering

[2] Tianjin University,The University Library

[3] Tianjin University,Department of Biochemical Engineering

来源：

Biotechnology Letters | 2001年 / 23卷

关键词：

GroEL; kinetics; lysozyme; model; renaturation;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

From the renaturation kinetics of denatured/reduced lysozyme assisted by the molecular chaperone GroEL, a simplified kinetic model was established based on the competition between protein folding and aggregation. In the presence of GroEL and ATP, the aggregate formation was a second order reaction. With 2 mM ATP, a renaturation yield of 90% at a high renaturation rate was obtained when the molar ratio of GroEL to lysozyme was 1:1.

引用

页码：1165 / 1169

页数：4

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