Biomechanical and histological comparison of the influence of oestrogen deficient state on tendon healing potential in rats

被引：0
作者：
Esra Circi
Sercan Akpinar
Cenk Balcik
Didem Bacanli
Gulnur Guven
Rahmi Can Akgun
Ismail Cengiz Tuncay
机构：
[1] Baskent University Faculty of Medicine,Department of Orthopaedics and Traumatology
[2] Baskent University Faculty of Engineering,Department of Mechanical Engineering
[3] Baskent University ,Experimental Research Center
[4] Baskent University Faculty of Medicine,Department of Pathology
[5] Baskent Universitesi Tip Fakultesi Hastanesi,Ortopedi ve Travmatoloji
来源：
International Orthopaedics | 2009年 / 33卷
关键词：
Proliferate Cell Nuclear Antigen; Achilles Tendon; Ultimate Load; Endogenous Oestrogen; Tendon Healing;
D O I：
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中图分类号：
学科分类号：
摘要：
Thirty-six female Sprague-Dawley rats were divided into two groups: oophrectomised (oestrogen deficient) rats and sham operated (oestrogen maintained) rats. Rats were sacrificed at six, ten, and 14 weeks. The rats were randomly chosen to have biomechanical evaluation on one side and histological evaluation on the other. Biomechanical testing was performed on an Instron machine to measure peak load. Histological sections were evaluated for cell proliferation, collagen-fibre organisation, fibroblast density, angiogenesis, inflammatory cells, chondroid and osseous metaplasia. Compared with the sham operated group, the oophrectomised group showed a lesser average maximum stress (42.9 N/m2 versus 33.7 N/m2) at six weeks, which was significant (p < .05). Succeeding weeks showed no significant biomechanical differences between the two groups. The sham operated group showed greater inflammatory response, which was statistically significant (p < 0.05), and also revealed greater cell proliferation and density. The results of this study revealed that endogenous oestrogen may improve healing of the Achilles tendon in rats.
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