Molecular detection of five cherry viruses from sweet cherry trees in Japan

被引：47

作者：

Isogai M. ^{[1
]}

Aoyagi J. ^{[1
]}

Nakagawa M. ^{[1
]}

Kubodera Y. ^{[1
]}

Satoh K. ^{[2
]}

Katoh T. ^{[2
]}

Inamori M. ^{[3
]}

Yamashita K. ^{[4
]}

Yoshikawa N. ^{[1
]}

机构：

[1] Plant Pathology Laboratory, Faculty of Agriculture, Iwate University, Morioka 020-8550

[2] Yamogata Hort. Experimental Station, Yamagata

[3] Ninohe Nougyou Kairyou Fukyu Center, Iwate

[4] Aomori Green Biocenter, Aomori

来源：

Journal of General Plant Pathology | 2004年 / 70卷 / 5期

关键词：

Cherry green ring mottle virus; Cherry necrotic rusty mottle virus; Cherry virus A; Little cherry virus-1; Little cherry virus-2;

D O I：

10.1007/s10327-004-0129-4

中图分类号：

学科分类号：

摘要：

To investigate the occurrence of viruses in sweet cherry in Japan, leaf samples were collected from sweet cherry trees in Aomori, Iwate, and Yamagata prefectures. Analysis of dsRNAs from sweet cherry leaves showed that 73% of 49 samples contained dsRNAs more than 6kbp in size. Reverse transcription- polymerase chain reaction analysis of cherry viruses indicated that 14% of the samples had Little cherry virus-1,65% had Little cherry virus-2,14% had Cherry necrotic rusty mottle virus, 49% had Cherry virus A, and 92% had Cherry green ring mottle virus. This is the first molecular detection of these five cherry viruses in Japan.

引用

页码：288 / 291

页数：3

共 20 条

[1] Eastwell K.C., Bernardy M.G., Partial characterization of a closterovirus associated with apple mealybug-transmitted little cherry disease in North America, Phytopathology, 91, pp. 268-273, (2001)
[2] Isogai M., Uyeda I., Kimura I., The genomic diversity of field isolates of rice black-streaked dwarf fijivirus, Ann Phytopathol Soc Jpn, 61, pp. 575-577, (1995)
[3] James D., Mukerji S., Mechanical transmission, identification, and characterization of a virus associated with mottle leaf in cherry, Plant Dis, 83, pp. 235-239, (1993)
[4] James D., Upton C., Single primer pair designs that facilitate simultaneous detection and differentiation of peach mosaic virus and cherry mottle leaf virus, J Virol Methods, 83, pp. 103-111, (1999)
[5] James D., Jelkmann W., Upton C., Nucleotide sequence and genome organization of cherry mottle leaf virus and its relationship to members of the Tricovirus genus, Arch Virol, 145, pp. 995-1007, (2000)
[6] James D., Howell W.E., Mink G.I., Molecular evidence of the relationship between a virus associated with flat apple disease and Cherry rasp leaf virus as determined by RT-PCR, Plant Dis, 85, pp. 47-52, (2001)
[7] Jelkmann W., Cherry virus A: cDNA cloning of dsRNA, nucleotide sequence analysis and serology reveal a new plant capillovirus in sweet cherry, J Gen Virol, 76, pp. 2015-2024, (1995)
[8] Jelkmann W., Fechtner B., Agranovsky A.A., Complete genome structure and phylogenetic analysis cherry virus, a mealybugtransmissible closterovirus, J Gen Virol, 78, pp. 2067-2071, (1997)
[9] Keim-Konrad R., Jelkman W., Genome analysis of the 3′-terminal part of the little cherry disease associated dsRNA reveals a monopartite clostero-like virus, Arch Virol, 141, pp. 1437-1451, (1996)
[10] Kikuchi S., Toshida K., Sato Y., Ishiguro M., Studies on cherry bud blight. 1. Research on actual condition and cause of sweet cherry bud blight, Bull Yamagata Hortic Exp Stn, 13, pp. 11-22, (2000)

← 1 2 →