Statistical medium formulation and process modeling by mixture design of experiment for peptide overexpression in recombinant Escherichia coli

被引:0
|
作者
Kwang-Min Lee
Chang-Hoon Rhee
Choong-Kyung Kang
Jung-Hoe Kim
机构
[1] Korea Advanced Institute of Science and Technology,Cellular Metabolic Engineering Lab.
[2] KoBioTech Co.,undefined
[3] Ltd.,undefined
来源
Applied Biochemistry and Biotechnology | 2006年 / 135卷
关键词
Anti-HIV peptide; statistical formulation; crossed design; robust process; propagation of error;
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学科分类号
摘要
The medium formulation and robust process modeling for anti-HIV peptide (T-20) production by recombinant Escherichia coli overexpression were studied by employing a crossed experimental design. The crossed design, a mixture design combined with process factor (induction duration), was used to find the optimal medium formulation and process time. The optimal settings for three major components, (7.75 mL of NPK sources, 5.5 mL of glucose, and 11.75 mL of MgSO4) characterized by %T-20 (14.45%), the proportion of peptide to the total protein, were observed in a total of 100 mL of medium inducted at an optical density of 0.67 with 0.7 mM isopropyl-β-d-thiogalactopyranoside) for a 3-h induction duration at shake-flask scale. These conditions were further investigated to find robust, process conditions (8.2 mL of NPK sources, 5.6 mL of glucose, and 11.3 mL of MgSO4, and a 3.5-h induction duration time) for T-20 production (13.9%) by applying propagation of error.
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页码:81 / 100
页数:19
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