Molecular characterization and prevalence of Cryptosporidium spp. in sheep and goats in western Inner Mongolia, China

被引：0
作者：
Jiashu Lang
Han Han
Heping Dong
Ziyang Qin
Yin Fu
Huikai Qin
Junchen Zhang
Jinfeng Zhao
Xiaoying Li
Guanghui Zhao
Junqiang Li
Longxian Zhang
机构：
[1] Henan Agricultural University,College of Veterinary Medicine
[2] International Joint Research Laboratory for Zoonotic Diseases of Henan,College of Veterinary Medicine
[3] Key Laboratory of Quality and Safety Control of Poultry Products (Zhengzhou),undefined
[4] Ministry of Agriculture and Rural Affairs,undefined
[5] Norman Bethune Health Science Center of Jilin University,undefined
[6] Northwest A&F University,undefined
来源：
Parasitology Research | 2023年 / 122卷
关键词：
Sheep; Goats; Zoonotic;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Cryptosporidium spp. are zoonotic intestinal parasites that infect fish, birds, reptiles and mammals. Cryptosporidium spp. are common cause of diarrhea. In this study, a total of 1032 fecal samples were collected from the rectums of sheep and goats. The samples were analyzed using nested polymerase chain reaction (nested PCR) based on the small subunit ribosomal RNA (SSU rRNA) gene of Cryptosporidium spp. The average infection rate of Cryptosporidium spp. was 2.23% (n = 23), and three Cryptosporidium species were identified, namely Cryptosporidium ubiquitum (8/23), Cryptosporidium andersoni (5/23) and Cryptosporidium xiaoi (10/23). Subtyping of C. ubiquitum and C. xiaoi was carried out by DNA sequence analysis of the 60-kDa glycoprotein (gp60) gene. Eight C. ubiquitum isolates were identified as zoonotic subtype XIIa. Nine C. xiaoi isolates were identified as subtypes XXIIIc (n = 1), XXIIIf (n = 3) and XXIIIg (n = 5). Subtype XXIIIg was first found in Chinese sheep. C. ubiquitum subtype XIIa was found in both sheep and goats, suggesting that sheep and goats are important sources of C. ubiquitum infections.
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页码：537 / 545
页数：8
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